Method for preventing chemical crosstalk in enzyme-linked reactions, and associated system
A method for biochemical analysis uses a micro-reaction array with at least two reaction chambers for materials which react together chemically or biochemically. The reaction chambers are smaller than 1 μl, the reaction chambers are filled together by throughflow, the chemical or biochemical reactions of the substances retained therein then occurs in the individual isolated reaction chambers, thus preventing an interference between the reactions in the individual reaction chambers and the reaction products remain enclosed in the relevant reaction chambers. In the system the planar array has at least two reaction chambers for substances, whereby the reaction chambers are closed with the goal of preventing an exchange of substances.
1. A method for inhibiting chemical crosstalk in enzyme-linked detection reactions, using a microreaction array with an array of reaction chambers for receiving substances which interact with one another, each reaction chamber having a respective volume, comprising:
connecting the reaction chambers to one another via a supply volume so as to at permit mass transfer between the reaction chambers;
selectively securing different enzyme labels in different reaction chambers such that different reaction chambers have a different enzyme label content;
allowing enzyme-linked reactions to proceed differently in the reaction chambers based on the different enzyme label content, the enzyme-linked reactions producing a product; and
closing the reaction chambers with a housing top part to inhibit chemical crosstalk, the housing top part disposed across openings in the reaction chambers to reduce the volume of the reaction chambers, and inhibit mass transfer of the product between the reaction chambers and the supply volume, in at least one direction,
wherein the reaction chambers are mechanically opened and closed to connect the reaction chambers and inhibit mass transfer between the reaction chambers, respectively, and
wherein the reaction chambers are closed off by the housing top part by means of a mechanical ram and opened by removing the housing top part.
2. The method as claimed in claim 1 , wherein at a first time, mass transfer takes place between the reaction chambers and the supply volume, and, at a second time, mass transfer between the reaction chambers is inhibited.
3. The method as claimed in claim 1 , wherein the reaction chambers and the supply volume contain different liquids having different solubility characteristics, and
mass transfer in one direction is selectively inhibited by the different solubility characteristics.
4. The method as claimed in claim 1 , wherein the reaction chambers are closed by displacement of the supply volume.
5. The method as claimed in claim 2 , wherein the reaction chambers are connected by temporarily creating permeable phase boundaries between a substance located in the reaction chambers and a substance located in the supply volume.
6. The method as claimed in claim 4 , wherein mass transfer is inhibited by displacement of the supply volume by a barrier medium.
7. The method as claimed in claim 6 , wherein
the supply volume is a liquid, and
the supply volume is displaced by a gas.
8. The method as claimed in claim 6 , wherein
the supply volume is a liquid, and
the supply volume is displaced by an immiscible liquid.
9. The method as claimed in claim 6 , wherein
the supply volume is a liquid, and
the supply volume is displaced by silicone oil.
10. The method as claimed in claim 3 , wherein
the reaction chambers contains a hydrogel layer,
starting materials for the reactions diffuse out of the supply volume into the hydrogel layer, and
the product does not diffuse out of the hydrogel layer.
11. The method as claimed in claim 1 , wherein a combinatorial analysis or synthesis of substances takes place in individual reaction chambers.
12. The method as claimed in claim 1 , wherein Redox recycling is performed with the enzyme-linked reactions.
13. The method as claimed in claim 2 , wherein the reaction chambers are mechanically opened and closed by a physical block to connect the reaction chambers and inhibit mass transfer, respectively.