Growth differentiation factor-11
View Patent ↗Growth differentiation factor-11 (GDF-11) is disclosed along with its polynucleotide sequence and amino acid sequence. The invention provides a method for identifying a compound that affects GDF-11 activity or gene expression.
1. A method for identifying a compound that affects GDF-11 activity or gene expression, comprising:
a) incubating the compound with a human GDF-11 polypeptide as set forth in SEQ ID NO: 2, or with a recombinant cell expressing a human GDF-11 as set forth in SEQ ID NO: 2 under conditions sufficient to allow the components to interact; and
b) determining the effect of the compound on GDF-11 activity or expression.
2. The method of claim 1 , wherein the effect is inhibition of GDF-11 activity or expression.
3. The method of claim 2 , wherein the effect is determined by comparing expression of GDF-11 prior to incubating with the compound with the expression of GDF-11 after incubating with the compound.
4. The method of claim 2 , wherein the effect is measured by Northern Blot analysis.
5. The method of claim 2 , wherein the effect is measured by Western Blot analysis.
6. The method of claim 2 , wherein the effect is measured by muscle fiber analysis.
7. The method of claim 2 , wherein the components interact in solution.
8. The method of claim 2 , wherein the components interact in solid phase.
9. The method of claim 2 , wherein the components interact in a cell.
10. The method of claim 2 , wherein the compound is a member of a combinatorial library.