Method of haplotype-based genetic analysis for determining risk for developing insulin resistance and coronary artery disease
View Patent ↗Disclosed is a method for determining haplotypes useful for large-scale genetic analysis, within a genomic reference sequence of interest, for a human subpopulation. The method can applied to statistically evaluating the genotypes of subjects for any statistically significant association with a phenotype of interest, such as insulin resistance or coronary artery disease. Thus, also disclosed are a method of detecting a genetic predisposition in a Mexican-American human subject for developing insulin resistance and methods of detecting a lower than normal risk in a Mexican-American human subject for developing insulin resistance or coronary artery disease.
1. A method of detecting a genetic predisposition in a Mexican-American human subject for developing insulin resistance, comprising:
a) collecting a biological sample from the subject;
b) genotyping the sample at nucleotide positions 7315, 8292, 8393, 8852, 9040, and 9712 SEQ ID NO: 25; and
c) assessing whether a haplotype is present in the sample, the haplotype comprising (nucleotide position: variant allele):
(i) 7315: G;
(ii) 8292: A;
(iii) 8393: G;
(iv) 8852: G;
(v) 9040: G; and
(vi) 9712: G,
wherein the presence of the haplotype indicates a genetic predisposition for developing insulin resistance in the subject.
2. A method of detecting a lower than normal risk in a Mexican-American human subject for developing insulin resistance, comprising:
a) collecting a biological sample from the subject;
b) genotyping the sample at nucleotide positions 7315, 8292, 8393, 8852, 9040 SEQ ID NO: 25; and
c) assessing whether a haplotype is present in the sample, the haplotype comprising (nucleotide position:variant allele):
(i) 7315: G;
(ii) 8292: A;
(iii) 8393: T;
(iv) 8852: T;
(v) 9040: C; and
(vi) 9712: G,
wherein the presence of the haplotype indicates a lower than normal risk for developing insulin resistance in the subject.
3. A method of detecting a lower than normal risk in a Mexican-American human subject for developing coronary artery disease, comprising:
a) collecting a biological sample from the subject;
b) genotyping the sample at nucleotide positions 7315, 8292, 8393, 8852, 9040 SEQ ID NO: 25; and
c) assessing whether the sample is homozygous for a haplotype comprising (nucleotide position: variant allele):
(i) 7315: G;
(ii) 8292: A;
(iii) 8393: T;
(iv) 8852: T;
(v) 9040: C; and
(vi) 9712: G,
wherein homozygosity for the haplotype indicates a lower than normal risk for developing coronary artery disease in the subject.