Fluorescence lifetime spectrometer (FLS) and methods of detecting diseased tissues
This invention relates to method and apparatus for collection of the complete fluorescence emission spectrum and the transient decay of a selected spectral band simultaneously with data acquisition times of less that one second. This invention is useful for studying human tissues and cells in vivo.
1. An apparatus for measuring spectral and temporal fluorescence properties of tissue and cells in vivo comprising:
(a) a primary excitation light source which is directed to a tissue or cell sample,
(b) an optical fiber probe which delivers light to the tissue or cell sample and collects resulting fluorescent emissions from said tissue or cell sample wherein said primary excitation light source is directed through the optical fiber probe to the tissue or cell sample;
(c) a means of directing the returning fluorescent emissions from the tissue or cell sample to spectral and temporal detection schemes;
(d) a spectrometer coupled ICCD which detects fluorescent emissions output from the tissue or cell sample and derives therefrom spectral fluorescence properties of the tissue or cell sample;
(e) an avalanche photodiode to detect temporal fluorescent emissions output from the tissue or cell sample; and
(f) an oscilloscope for transiently digitizing a signal from the avalanche photodiode and deriving temporal fluorescence properties of the tissue or cell sample from the signal, said oscilloscope being connected to the avalanche photodiode.