Polypeptides of
The present invention relates to polypeptides of Pseudomonas aeruginosa which may be used to prevent, diagnose and/or treat Pseudomonas aeruginosa infection.
1. An isolated polypeptide consisting of the amino acid sequence set forth as SEQ ID NO:4 from which the signal peptide sequence set forth as SEQ ID NO:38 is deleted, wherein the polypeptide is capable of eliciting an immune response in a host to Pseudomonas aeruginosa.
2. A pharmaceutical composition comprising a pharmaceutically acceptable carrier or diluent and an amount of a polypeptide that is capable of eliciting an immune response in a host to Pseudomonas aeruginosa , wherein the polypeptide consists of an amino acid sequence at least 95% identical to the amino acid sequence set forth as SEQ ID NO:4, and wherein the polypeptide is capable of inducing an antibody that binds specifically to a SPA-2 polypeptide consisting of the amino acid sequence set forth as SEQ ID NO:4.
3. A pharmaceutical composition comprising a polypeptide and a liposome, wherein the polypeptide consists of an amino acid sequence at least 95% identical to the amino acid sequence set forth as SEQ ID NO:4, and wherein the polypeptide is capable of eliciting an immune response in a host to Pseudomonas aeruginosa , and wherein the polypeptide is capable of inducing an antibody that binds specifically to a SPA-2 polypeptide consisting of the amino acid sequence set forth as SEQ ID NO:4.
4. The pharmaceutical composition of claim 2 wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO:4 from which the signal peptide sequence set forth as SEQ ID NO:38 is deleted.
5. The pharmaceutical composition of claim 2 wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO:4.
6. The pharmaceutical composition of claim 3 wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO:4 from which the signal peptide sequence set forth as SEQ ID NO:38 has been deleted.
7. The pharmaceutical composition of claim 3 wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO:4.
8. The pharmaceutical composition according to claim 2 further comprising a pharmaceutically acceptable adjuvant.
9. The pharmaceutical composition according to claim 3 further comprising a pharmaceutically acceptable adjuvant.
10. A kit comprising a polypeptide for detection or diagnosis of Pseudomonas aeruginosa infection, wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO:4 from which the signal peptide sequence set forth as SEQ ID NO:38 is deleted.