Vector mediated organelle transfection
The present invention provides compositions and methods for direct transfection of mitochondria and chloroplast DNA in living cells. More particularly, the present invention is based on the use of viral vectors that specifically bind to receptors uniquely found on the target organelle. In one embodiment, as shown in FIG. 1 , a eukaryotic cell containing an organelle ( 1 ) that has been modified to express a viral receptor ( 2 ) on the organelle's surface is provided. A viral vector ( 6 ) comprising a desired recombinant DNA construct ( 3 ) is introduced into the cytosol of the cell, wherein the viral vector binds to its receptor and introduces the recombinant DNA into the interior of the organelle.
1. An isolated nucleic acid comprising a sequence encoding a mitochondrial localization signal from subunit VIII of human cytochrome oxidase operably linked to a sequence encoding a viral receptor, wherein the viral receptor is expressed on an outer mitochondrial membrane after the nucleic acid has been transfected into a eukaryotic cell.
2. The nucleic acid of claim 1 wherein the viral receptor is selected from the group consisting of OmpF, OmpC, PhoE and lamB.
3. The nucleic acid of claim 2 wherein the viral receptor is lamB.
4. An isolated eukaryotic cell comprising the nucleic acid of claim 1 .