Alleles of the sigA gene from coryneform bacteria
The invention relates to alleles of the sigA gene from coryneform bacteria which code for sigma factors A and a process for the fermentative preparation of L-lysine using bacteria which contain these alleles.
1. An isolated protein comprising the amino acid sequence of SEQ ID NO:2 and wherein:
a) the amino acid at position 414 of said protein is L-valine; and
b) said protein has sigma factor A biological activity.
2. The protein of claim 1 , wherein said amino acid sequence consists of the amino acid sequence of SEQ ID NO:2 but wherein the amino acid at position 414 is L-valine.
3. An isolated nucleic acid comprising a nucleotide sequence coding for the protein of either claim 1 or claim 2 .
4. The isolated nucleic acid 3 wherein said isolated nucleic acid comprises the nucleotide sequence of SEQ ID NO:3.
5. A vector comprising the nucleotide sequence of the isolated nucleic acid of claim 3 .
6. A coryneform bacteria transformed with the vector of claim 5 .
7. A process for the production of L-lysine or of a feed additive containing L-lysine, comprising:
a) fermenting the coryneform bacteria of claim 6 under conditions suitable for the production of L-lysine wherein said fermenting produces a fermentation liquor, and
b) isolating said L-lysine or the fermentation liquor containing said L-lysine.
8. The process of claim 7 , wherein at least one gene of the biosynthesis pathway of L-lysine is additionally overexpressed in said coryneform bacteria.
9. The process of claim 7 , wherein the activity of at least one metabolic pathway that reduces the formation of L-lysine is attenuated in said coryneform bacteria.
10. The process of claim 7 , wherein said composition of L-lysine or said fermentation liquor comprises >0 to 100% of the constituents of said fermentation liquor and/or of the biomass present during fermentation.
11. An isolated nucleic acid consisting of a nucleotide sequence coding for the protein of either claim 1 or claim 2 .
12. The isolated nucleic acid of claim 11 , wherein said isolated nucleic acid consists of the nucleotide sequence of SEQ ID NO:3.
13. A vector comprising the nucleotide sequence of the isolated nucleic acid of claim 11 .
14. A coryneform bacteria transformed with the vector of claim 13 .
15. A process for the production of L-lysine or of a feed additive containing L-lysine, comprising:
a) fermenting the coryneform bacteria of claim 14 under conditions suitable for the production of L-lysine, wherein said fermenting produces a fermentation liquor, and
b) isolating said L-lysine or the fermentation liquor containing said L-lysine.
16. The process of claim 15 , wherein at least one gene of the biosynthesis pathway of L-lysine is additionally overexpressed in said coryneform bacteria.
17. The process of claim 15 , wherein the activity of at least one metabolic pathway that reduces the formation of L-lysine is attenuated in said coryneform bacteria.