Composition and process for stabilizing of biomolecules
The present invention relates in general to the field of biotechnology. The invention particularly relates to a composition and process for stabilizing or preserving biological molecules, as well as devices that comprise correspondingly stabilized or preserved biomolecules.
1. A process for stabilizing or preserving a biomolecule comprising the steps of:
(a) providing a biomolecule immobilized on a surface; and
(b) covering the surface with a composition comprising:
i. at least one non-reducing disaccharide selected from the group consisting of trehalose (D-glucopyranosyl-D-glucopyranoside), sucrose (β-D-fructofuranosyl-α-D-glucopyranoside), and a derivative thereof; and
ii. at least one protein or polypeptide of the Late Embryogenesis Abundant (LEA) class comprising a motif comprising 11 amino acids, wherein the motif is selected from the group consisting of:
(a) K-T-A-E-F-R-D-S-A-G-E (SEQ ID NO. 2),
(b) K-G-Q-E-F-K-E-R-A-G-E (SEQ ID NO. 3),
(c) K-A-E-E-T-K-Q-R-A-G-E (SEQ ID NO. 4),
(d) K-M-D-E-T-K-Q-R-A-G-E (SEQ ID NO. 5),
(e) K-A-R-K-T-K-D-S-A-A-E (SEQ ID NO. 6),
(f) K-A-K-E-Y-K-D-Y-T-A-E (SEQ ID NO. 7),
(g) K-A-R-E-T-T-E-K-A-R-E (SEQ ID NO. 8),
and
(h) T-K-D-S-A-A-E-K-A-R-E (SEQ ID NO. 9).
2. A process according to claim 1 , wherein said non-reducing disaccharide is present at from 0.01 to 15 weight percent in relation to a ready-to-use solution and said protein or polypeptide of the LEA class is present at from 0.00001 to 1 weight percent in relation to the ready-to-use solution.
3. A process for the production of a surface with an immobilized and stabilized or preserved biomolecule, comprising the steps of:
(a) providing a surface with a biomolecule immobilized thereon; and
(b) covering the biomolecule with a composition comprising:
i. at least one non-reducing disaccharide selected from the group consisting of trehalose (D-glucopyranosyl-D-glucopyranoside), sucrose (β-D-fructofuranosyl-α-D-glucopyranoside), and a derivative thereof; and
ii. at least one protein or polypeptide of the (LEA) class comprising a motif comprising 11 amino acids, wherein the motif is selected from the group consisting of:
(a) K-T-A-E-F-R-D-S-A-G-E (SEQ ID NO. 2),
(b) K-G-Q-E-F-K-E-R-A-G-E (SEQ ID NO. 3),
(c) K-A-E-E-T-K-Q-R-A-G-E (SEQ ID NO. 4),
(d) K-M-D-E-T-K-Q-R-A-G-E (SEQ ID NO. 5),
(e) K-A-R-K-T-K-D-S-A-A-E (SEQ ID NO. 6),
(f) K-A-K-E-Y-K-D-Y-T-A-E (SEQ ID NO. 7),
(g) K-A-R-E-T-T-E-K-A-R-E (SEQ ID NO. 8),
and
(h) T-K-D-S-A-A-E-K-A-R-E (SEQ ID NO. 9).
4. A surface of a material selected from the group consisting of glass, quartz glass, quartz, silicon, polymers, nitrocellulose, nylon and micro fiber membranes, and paper, wherein the surface includes a biomolecule immobilized thereon, covered with a stabilizing or preserving composition comprising:
at least one non-reducing disaccharide selected from the group consisting of trehalose (D-glucopyranosyl-D-glucopyranoside), sucrose (β-D-fructofuranosyl-α-D-glucopyranoside), and a derivative thereof; and
at least one protein or polypeptide of the LEA class comprising a motif comprising 11 amino acids, wherein the motif is selected from the group consisting of:
(a) K-T-A-E-F-R-D-S-A-G-E
(SEQ ID NO. 2),
(b) K-G-Q-E-F-K-E-R-A-G-E
(SEQ ID NO. 3),
(c) K-A-E-E-T-K-Q-R-A-G-E
(SEQ ID NO. 4),
(d) K-M-D-E-T-K-Q-R-A-G-E
(SEQ ID NO. 5),
(e) K-A-R-K-T-K-D-S-A-A-E
(SEQ ID NO. 6),
(f) K-A-K-E-Y-K-D-Y-T-A-E
(SEQ ID NO. 7),
(g) K-A-R-E-T-T-E-K-A-R-E
(SEQ ID NO. 8), and
(h) T-K-D-S-A-A-E-K-A-R-E
(SEQ ID NO. 9).
5. A surface according to claim 4 , wherein said non-reducing disaccharide is present at from 0.01 to 15 weight percent in relation to a ready-to-use solution and said protein or polypeptide of the LEA class is present at from 0.00001 to 1 weight percent in relation to the ready-to-use solution.
6. An analytical and/or diagnostic device, comprising a surface as defined in claim 4 .
7. A device according to claim 6 selected from the group consisting of biochips, sensor chips, microtiter plates, test tubes and culture dishes.