Mesoporous platinum electrode and method for detecting biochemical substrate using the mesoporous platinum electrode
The present invention relates to a mesoporous platinum electrode for detecting biochemical substrate, comprising an electrode and a mesoporous platinum layer covering the surface thereof, and a method for detecting a biochemical substrate using the mesoporous platinum electrode. Using the present invention, glucose concentration can be selectively determined while excluding interference of interfering agents.
1. A method for detecting glucose comprising:
obtaining a mesoporous platinum electrode in which the mesoporous platinum electrode comprises an electrode and a mesoporous platinum layer covering the surface thereof and in which the mesoporous platinum electrode is a non-enzymatic electrode without any enzyme immobilized thereon that reacts with the glucose to produce an electrical signal; contacting a sample solution expected to contain the biochemical substrate with the mesoporous platinum electrode; detecting a response current generated by applying a voltage to the mesoporous platinum electrode.
2. The method of claim 1 , wherein the electrode is a noble metal or an acid-resistive metal.
3. The method of claim 1 , wherein the electrode is selected from the group consisting of carbon, platinum, gold, silver, and stainless steel.
4. The method of claim 1 , wherein the mesoporous platinum layer has a thickness of 20-5000 nm.
5. The method of claim 1 , wherein the current is measured amperometrically.
6. The method of claim 1 , wherein a range of the applying voltage is between −0.1 and 0.5 V vs. a reference electrode.
7. The method of claim 6 , wherein the reference electrode is Ag/AgCl.
8. The method of claim 1 , wherein the current generated is proportional to the glucose present in the sample from a range of 0 to 20 mM glucose.