Immunotherapy in cancer treatment
The present invention relates to a novel method of cancer immuno-therapy and to a kit for use in this method. Specifically, the present invention relates to a novel method of collecting lymphocytes from sentinel lumph nodes and in vitro culture for the multiplication thereof lymphocytes.
1. A method for treating cancer having a primary tumor in a human patient, comprising
(a) providing lymphocytes obtained from sentinel lymph nodes from the patient, wherein the sentinel lymph nodes are the first lymph nodes in the lymphatic system that receive lymphatic drainage from the primary tumor area;
(b) expanding the lymphocytes in vitro, wherein the lymphocytes are stimulated in vitro by addition of a stimulating agent selected from the group consisting of IL-2, IL-12, anti-CD3 antibody, and anti-CD28 antibody and are exposed to recombinant tumor antigen and/or autologous tumor extract, optionally presented with antigen presenting cells selected from B-cells, macrophages, monocytes, dendritic cells, or Langerhans cells; and
(c) transferring the expanded lymphocytes into the patient.
2. The method of claim 1 , wherein the lymphocytes are stimulated with IL-2.
3. The method of claim 1 , wherein the lymphocytes are stimulated with anti-CD3 antibody.
4. The method of claim 1 , wherein the lymphocytes are stimulated with anti-CD28 antibody.
5. The method of claim 1 , wherein the lymphocytes are first exposed to autologous tumor extract and then are stimulated with IL-2.
6. The method of claim 1 , wherein the lymphocytes are exposed to recombinant tumor antigen.
7. The method of claim 1 , wherein the lymphocytes are exposed to autologous tumor extract.
8. The method of claim 1 , wherein the sentinel lymph nodes are free of tumor cells.
9. The method of claim 1 , wherein the lymphocytes are separated from the stimulating agent after stimulation.
10. The method of claim 9 , wherein the lymphocytes are separated from recombinant tumor antigen and/or autologous tumor extract after exposure.
11. The method of claim 9 , wherein after separation, the lymphocytes are restimulated by a further addition of the stimulating agent.
12. The method of claim 11 , wherein the further addition of the stimulating agent is made with addition of medium to a culture of the lymphocytes.
13. The method of claim 12 , wherein the lymphocytes are restimulated with IL-2.
14. The method of claim 9 , wherein after separation, the lymphocytes are restimulated by a further addition of IL-2.