Treatment of metastatic colon cancer with b-subunit of shiga toxin
The present invention relates to compositions and methods for treating invasive or metastatic cancer, particularly metastatic colon cancer using the B-submit of shiga toxin. The invention further provides methods of identifying compounds usefully for the treatment of invasive or metastatic cancer, particularly metastatic colon cancer.
1. A method of reducing, or inhibiting invasiveness and metastasis of tumor cells in a subject, wherein the tumor cells produce Gb 3 , comprising administering to the subject a therapeutically effective amount of a Stx1B-subunit of Shiga toxin.
2. The method of claim 1 , wherein the tumor cells are colon tumor cells.
3. The method of claim 1 , wherein the tumor cells are from a tissue selected from the group consisting of: colon, lung, brain, skin, ovary, pancreas, liver, stomach, bladder, bone, testicle, uterus, adipose tissue, throat, kidney, tongue, pituitary gland, thyroid, lymphoid tissue, eye, and cervix.
4. The method of claim 1 , wherein the therapeutically effective amount of the Stx1B-subunit of Shiga toxin is administered prior to the onset of metastasis by the tumor cells.
5. The method of claim 1 , wherein the therapeutically effective amount of the B-subunit of Shiga toxin is administered subsequent to the onset of metastasis by the tumor cells.
6. The method of claim 1 , further comprising administering to the subject a therapeutically effective amount of radiation.
7. The method of claim 1 , further comprising administering to the subject a therapeutically effective amount of at least one chemotherapeutic agent.
8. The method of claim 1 , wherein the subject is a human.
9. The method of claim 1 , wherein the Stx1B-subunit of Shiga toxin is conjugated to a therapeutic moiety.
10. A method of reducing, or inhibiting invasiveness and metastasis of colon tumor cells in a subject, wherein the tumor cells produce Gb 3 , comprising administering to the subject a therapeutically effective amount of a Stx1B-subunit of Shiga toxin.