Enzymes and polynucleotides encoding the same
View Patent ↗The invention relates to a multifunctional germacrene-D synthase and a polynucleotide encoding it. Included also are genetic constructs, transgenic organisms, plant selection methods and biofermentation methods. The enzyme may be used to prepare germacrene-D and/or other sesquiterpenes such as germacrene-D, delta-cadinene, gamma-cadinene, gamma-muurolene, gamma-elemene, delta-elemene, elemol or germacrene-B.
1. An isolated polynucleotide encoding a multifunctional germacrene-D synthase, wherein the polynucleotide sequence is set forth in SEQ ID NO:1.
2. A genetic construct comprising the polynucleotide of claim 1 .
3. A vector comprising a genetic construct of claim 2 .
4. An isolated polynucleotide encoding a multifunctional germacrene-D synthase having the amino acid sequence of SEQ ID NO:2.
5. A genetic construct comprising the polynucleotide of claim 4 .
6. The genetic construct of claim 5 further comprising a promoter sequence.
7. The genetic construct of claim 6 further comprising a termination sequence.
8. A vector comprising a genetic construct of claim 5 .