Texturizing lactic bacteria
View Patent ↗The invention relates to a novel strain of lactic bacteria comprising at least one sequence selected from a group consisting of nucleotide sequences SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8 and to a method for the production of said strains. The invention also relates to the sequences SEQ ID Nos. 1-8 and to a nucleic acid, vectors and plasmids comprising said sequences. The invention further relates to food products, especially milk products containing said strains.
1. An isolated strain of bacterium comprising at least one sequence selected from the group constituted by the nucleotide sequences SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6, SEQ ID No.7, and SEQ ID No. 8.
2. The bacterium according to claim 1 wherein the bacterium is a texturizing strain of lactic bacterium characterized in that it is chosen from the genera Streptococcus, Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Bifidobacterium.
3. The bacterium according to claim 1 characterized in that it belongs to the species Streptococcus thermophilus.
4. A Streptococcus thermophilus deposited on 26th Feb. 2003 at the Collection Nationale de Cultures de Microorganismes under number I-2980.
5. An isolated polynucleotide comprising the sequence as set forth in SEQ ID No. 1.
6. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 2.
7. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 3.
8. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 4.
9. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 5.
10. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 6.
11. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 7.
12. An isolated polynucleotide sequence comprising the sequence SEQ ID No. 8.
13. An isolated polynucleotide comprising at least one sequence selected from the group constituted by the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No, 6, SEQ ID No.7, and SEQ ID No.8.
14. Cloning and/or expression vector comprising at least one nucleic acid according to claim 13 .
15. Vector according to claim 14 characterized in that it is a plasmid.
16. Host bacterium transformed by a vector according to claim 14 .
17. Bacterium according to claim 16 characterized in that it is a lactic bacterium.
18. Bacterium according to claim 16 characterized in that it is a lactic bacterium chosen from the genera Streptococcus, Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Bifidobacterium.
19. Process for constructing a strain according to claim 1 characterized in that the strain or the bacterium are obtained by transformation using a vector comprising at least one sequence selected from the group constituted by the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6, SEQ ID No.7, SEQ ID NO.8 or a nucleic acid comprising at least one sequence constituted by the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No, 6, SEQ ID No.7, and SEQ ID No. 8.
20. Process according to claim 19 characterized in that the transformation is followed by an insertion into the genome of the strain or of the host bacterium by at least one recombination event.
21. Process according to claim 19 characterized in that the vector is a plasmid.
22. Bacterial composition comprising at least one strain according to claim 1 .
23. Food or pharmaceutical composition comprising at least the strain according to the claim 1 .
24. Dairy product comprising at least the strain according to claim 1 .
25. Dairy product according to claim 24 characterized in that it is a fermented milk, a yogurt, a matured cream, a cheese, a fromage frais, a milk beverage, a dairy product retentate, a process cheese, a cream dessert, a cottage cheese or infant milk.
26. Dairy product according to claim 24 characterized in that it comprises milk of animal and/or plant origin.
27. Process for constructing a bacterium according to claim 16 characterized in that the strain or the bacterium are obtained by transformation using a vector comprising at least one sequence selected from the group constituted by the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6, SEQ ID No.7, SEQ ID NO.8 or a nucleic acid comprising at least one sequence constituted by the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No, 6, SEQ ID No.7, and SEQ ID No. 8.
28. Bacterial composition comprising at least one transformed bacterium according to claim 16 .
29. Food or pharmaceutical composition comprising the bacterial composition according to claim 22 .
30. Dairy product comprising the bacterial composition according to claim 22 .
31. A method for fermenting a dairy product comprises using a bacterium culture selected from the group consisting of Streptococcus, Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Bifidobacterium containing recombinant DNA comprising at least one sequence selected from the group consisting of the nucleotide sequences SEQ ID No. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No, 6, SEQ ID No.7, and SEQ ID No.8.
32. The method of claim 31 wherein the DNA is in a vector which was used to transform the culture.
33. The bacterial composition of claim 28 wherein the composition is a mixture of strains selected from the group consisting of mixtures of Streptococcus thermophilus with other Streptococcus thermophilus , mixtures of Streptococcus thermophilus with Lactobacillus delbrueckii subsp. Bulgaricus , mixtures of Streptococcus thermophilus with other Lactobacillus and/or with Bifidobacterium , mixtures of Streptococcus thermophilus with Lactococcus , and mixtures of Streptococcus thermophilus with other strains of lactic bacteria and yeasts.
34. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.1.
35. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.2.
36. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.3.
37. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.4.
38. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.5.
39. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.6.
40. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.7.
41. An isolated strain of bacterium according to claim 1 comprising the nucleotide sequence SEQ ID No.8.