Composite porous membrane and process for producing the same
There is provided a composite porous membrane comprising a porous membrane comprised of an organic polymeric compound, and a supporting porous membrane adjacent to the porous membrane, characterized in that the organic polymeric compound constituting the porous membrane penetrates in at least part of a surface adjacent to porous membrane of the supporting porous membrane, the porous membrane having specified opening ratio, average pore diameter, standard deviation of pore diameter, ratio of through pore, average membrane thickness, standard deviation of membrane thickness and internal structure, and that the supporting porous membrane has communicating pores of 0.5 D μm or greater average pore diameter. Further, there are provided a blood filtration membrane comprising the composite porous membrane; a leukocyte removing filter unit comprising the composite porous membrane as a second filter; and, utilizing the composite porous membrane, a cell culturing diaphragm and method of cell culturing.
1. A process for co-culturing cells of a first cell group and cells of a second cell group while partitioning the first and second cell groups by a cell culture diaphragm in a cell culture solution so that the cells of the two kinds of cell groups can come into contact with each other,
said cell culture diaphragm comprising a composite porous membrane which comprises at least one porous membrane comprising an organic polymer and at least one supporting porous membrane adjacent thereto, the supporting porous membrane having continuous pores with an average pore diameter between 1 and 100 μm, and a density of 0.1 to 0.5 g/cm 3 ,
wherein, when a membrane flat surface of the porous membrane is observed using a photomicrograph, the porous membrane has an opening ratio between 10% and 90%, an average pore diameter D (μm) of 0.1≦D≦50, a standard deviation σd (μm) of pore diameter of 0≦σd/D≦0.6, and the percentage of through-pores to all the pores of the porous membrane is 30% or more; and when a membrane section thereof is observed using a photomicrograph, the porous membrane has an average membrane thickness T (μm) defined by 0.05≦T/D≦2 and a structure in which pores adjacent to one another communicate with one another therein;
the process comprising:
inoculating the cells of the first cell group on the side of the supporting porous membrane,
inoculating the cells of the second cell group on the side of the porous membrane, and
co-culturing the cells of the first and second cell groups while laying the side of the porous membrane up.
2. The method according to claim 1 , wherein the porous membrane has an opening ratio between 15% and 80% and an average pore diameter D (μm) of 0.5≦D≦20.
3. The method according to claim 1 , wherein the porous membrane has an average membrane thickness T (μm) of 0.1≦T≦50.
4. The method according to claim 1 , wherein the porous membrane has an average pore diameter D (μm) of 0.1≦D≦20 and an average membrane thickness T (μm) of 0.1≦T≦20, and wherein a standard deviation σt (μm) of the membrane thickness is defined by 0≦σt/T≦0.5.
5. The method according to claim 4 , wherein the composite porous membrane further comprises a nonwoven fabric adjacent to the porous membrane, and the nonwoven fabric has an average pore diameter between 1 and 100 μm.