Fungal proteins and nucleic acids encoding same
Disclosed herein are fungal nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The novel leucine aminopeptidase (LAP) and other amino- and carboxypeptidases polypeptides, referred to herein as EXOX nucleic acids and proteins of the invention are useful in a variety of medical, research, and commercial applications.
1. An enzyme cocktail comprising
a) a dipeptidyl peptidase IV (DPP IV) polypeptide comprising an amino acid sequence which is at least 95% identical to SEQ ID NO: 35, or a fragment thereof, wherein said fragment has dipeptidyl peptidase IV activity, and
b) a leucine aminopeptidase (LAP) polypeptide comprising an amino acid sequence which is at least 95% identical to SEQ ID NO: 6, or a fragment thereof, wherein said fragment has leucine aminopeptidase activity.
2. The enzyme cocktail of claim 1 , wherein the LAP is obtained from a dermatophyte selected from the group consisting of Epidermophyton floccosum, Microsporum audouinii, Microsporum ferrugineum, Trichophyton concentricum, Trichophyton kanei, Trichophyton megninii, Trichophyton mentagrophytes, Trichophyton raubitschekii, Trichophyton schoenleinii, Trichophyton soudanense, Trichophyton tonsurans, Trichophyton violaceum, Trichophyton yaoundei, Microsporum canis, Microsporum equinum, Microsporum nanum, Microsporum persicolor, Trichophyton equinum, Trichophyton simii, Trichophyton verrucosum, Microsporum gypseum, Trichophyton ajelloi , and Trichophyton terrestre.
3. The enzyme cocktail of claim 1 , further comprising one or more proteases.
4. The enzyme cocktail of claim 3 , wherein said one or more proteases is trypsin.
5. A kit comprising, in one or more containers, the enzyme cocktail of claim 1 .
6. The enzyme cocktail of claim 1 , wherein the DPPIV comprises the amino acid sequence set forth in SEQ ID NO: 35.
7. The enzyme cocktail of claim 1 , wherein the DPPIV consists of the amino acid sequence set forth in SEQ ID NO: 35.
8. The enzyme cocktail of claim 1 , wherein the DPPIV comprises an amino acid sequence comprising conservative amino acid substitutions of less than 5% of the amino acid residues of SEQ ID NO: 35.
9. The enzyme cocktail of claim 1 , wherein the LAP comprises the amino acid sequence set forth as SEQ ID NO:6.
10. The enzyme cocktail of claim 1 , wherein the LAP consists of the amino acid sequence set forth as SEQ ID NO:6.
11. The enzyme cocktail of claim 1 , wherein the LAP comprises an amino acid sequence comprising conservative amino acid substitutions of less than 5% of the amino acid residues of SEQ ID NO: 6.
12. The enzyme cocktail of claim 8 , wherein the conservative amino acid substitution comprises substitution of
i) lysine, arginine, or histidine, for each other;
ii) aspartic acid or glutamic acid for each other;
iii) glycine, asparagine, glutamine, serine, threonine, tyrosine, or cysteine for each other;
iv) alanine, valine, leucine, isoleucine, proline, hydroxyproline, phenylalanine, methionine, or tryptophan for each other;
v) threonine, valine, or isoleucine for each other; or
vi) tyrosine, phenylalanine, tryptophan, or histidine for each other.
13. The enzyme cocktail of claim 11 , wherein the conservative amino acid substitution comprises substitution of
i) lysine, arginine, or histidine, for each other;
ii) aspartic acid or glutamic acid for each other;
iii) glycine, asparagine, glutamine, serine, threonine, tyrosine, or cysteine for each other;
iv) alanine, valine, leucine, isoleucine, proline, hydroxyproline, phenylalanine, methionine, or tryptophan for each other;
v) threonine, valine, or isoleucine for each other; or
vi) tyrosine, phenylalanine, tryptophan, or histidine for each other.
14. The enzyme cocktail of claim 3 , wherein said one or more proteases is pronase.
15. The enzyme cocktail of claim 3 , wherein said one or more proteases is chymotrypsin.
16. The enzyme cocktail of claim 3 , wherein said one or more proteases is proteinase K.
17. The enzyme cocktail of claim 1 , wherein the LAP is obtained from Trichophyton rubrum.
18. An enzyme cocktail comprising
a) a dipeptidyl peptidase IV (DPP IV) polypeptide comprising an amino acid sequence which is at least 95% identical to SEQ ID NO: 35, or a fragment thereof, wherein said fragment has dipeptidyl peptidase IV activity, and
b) a leucine aminopeptidase (LAP) polypeptide comprising an amino acid sequence which is at least 95% identical to SEQ ID NO: 3, or a fragment thereof, wherein said fragment has leucine aminopeptidase activity.
19. The enzyme cocktail of claim 18 , wherein the LAP comprises the amino acid sequence set forth as SEQ ID NO: 3.
20. The enzyme cocktail of claim 18 , wherein the LAP comprises an amino acid sequence set forth as SEQ ID NO: 33.
21. The enzyme cocktail of claim 18 , wherein the LAP consists of the amino acid sequence set forth as SEQ ID NO: 3.
22. The enzyme cocktail of claim 18 , wherein the LAP comprises an amino acid sequence comprising conservative amino acid substitutions of less than 5% of the amino acid residues of SEQ ID NO: 3.
23. The enzyme cocktail of claim 18 , wherein the DPPIV comprises the amino acid sequence set forth in SEQ ID NO: 35.
24. The enzyme cocktail of claim 18 , wherein the DPPIV consists of the amino acid sequence set forth in SEQ ID NO: 35.
25. The enzyme cocktail of claim 18 , wherein the DPPIV comprises an amino acid sequence comprising conservative amino acid substitutions of less than 5% of the amino acid residues of SEQ ID NO: 35.
26. The enzyme cocktail of claim 25 , wherein the conservative amino acid substitution comprises substitution of
i) lysine, arginine, or histidine, for each other;
ii) aspartic acid or glutamic acid for each other;
iii) glycine, asparagine, glutamine, serine, threonine, tyrosine, or cysteine for each other;
iv) alanine, valine, leucine, isoleucine, proline, hydroxyproline, phenylalanine, methionine, or tryptophan for each other;
v) threonine, valine, or isoleucine for each other; or
vi) tyrosine, phenylalanine, tryptophan, or histidine for each other.
27. The enzyme cocktail of claim 22 , wherein the conservative amino acid substitution comprises substitution of
i) lysine, arginine, or histidine, for each other;
ii) aspartic acid or glutamic acid for each other;
iii) glycine, asparagine, glutamine, serine, threonine, tyrosine, or cysteine for each other;
iv) alanine, valine, leucine, isoleucine, proline, hydroxyproline, phenylalanine, methionine, or tryptophan for each other;
v) threonine, valine, or isoleucine for each other; or
vi) tyrosine, phenylalanine, tryptophan, or histidine for each other.
28. The enzyme cocktail of claim 18 , further comprising a second leucine aminopeptidase (LAP) polypeptide comprising an amino acid sequence which is at least 95% identical to SEQ ID NO: 6, or a fragment thereof, wherein said fragment has leucine aminopeptidase activity.
29. The enzyme cocktail of claim 28 , wherein the second LAP comprises the amino acid sequence set forth as SEQ ID NO: 6.
30. The enzyme cocktail of claim 28 , wherein the second LAP consists of the amino acid sequence set forth as SEQ ID NO: 6.
31. The enzyme cocktail of claim 28 , wherein the second LAP comprises an amino acid sequence comprising conservative amino acid substitutions of less than 5% of the amino acid residues of SEQ ID NO: 6.
32. The enzyme cocktail of claim 31 , wherein the conservative amino acid substitution comprises substitution of
i) lysine, arginine, or histidine, for each other;
ii) aspartic acid or glutamic acid for each other;
iii) glycine, asparagine, glutamine, seine, threonine, tyrosine, or cysteine for each other;
iv) alanine, valine, leucine, isoleucine, proline, hydroxyproline, phenylalanine, methionine, or tryptophan for each other;
v) threonine, valine, or isoleucine for each other; or
vi) tyrosine, phenylalanine, tryptophan, or histidine for each other.
33. A kit comprising, in one or more containers, the enzyme cocktail of claim 18 .
34. The enzyme cocktail of claim 18 , wherein the LAP is obtained from a dermatophyte selected characterized in that the dermatophyte is selected from the group consisting of Epidermophyton floccosum, Microsporum audouinii, Microsporum ferrugineum, Trichophyton concentricum, Trichophyton kanei, Trichophyton megninii, Trichophyton mentagrophytes, Trichophyton raubitschekii, Trichophyton schoenleinii, Trichophyton soudanense, Trichophyton tonsurans, Trichophyton violaceum, Trichophyton yaoundei, Microsporum canis, Microsporum equinum, Microsporum nanum, Microsporum persicolor, Trichophyton equinum, Trichophyton simii, Trichophyton verrucosum, Microsporum gypseum, Trichophyton ajelloi , and Trichophyton terrestre.
35. The enzyme cocktail of claim 18 , wherein the LAP is obtained from Trichophyton rubrum.
36. The enzyme cocktail of claim 18 , further comprising one or more proteases.
37. The enzyme cocktail of claim 36 , wherein said one or more proteases is trypsin.
38. The enzyme cocktail of claim 36 , wherein said one or more proteases is pronase.
39. The enzyme cocktail of claim 36 , wherein said one or more proteases is chymotrypsin.
40. The enzyme cocktail of claim 36 , wherein said one or more proteases is proteinase K.