Isolated nucleic acid molecules from the genome of citrus sudden death virus and uses thereof
The present disclosure relates to nucleic acid molecules comprising the genome of the Citrus Sudden Death Virus (CSDV), the causative agent of Citrus Sudden Death (CSD) disease. The cloned CSDV nucleic acid molecules can be used as probes or can be used to design oligonucleotide primers useful in assays, such as a polymerase chain reaction, for detecting the presence of CSDV in biological samples, particularly leaves, roots and other tissues or organs of plants, such as plants from the genera Citrus and Poncirus . The disclosure also comprises the nucleic CSDV nucleic acid molecules, in whole or part, as well as transgenic plants, such as monocots and dicots, containing the CSDV nucleic acid molecules, in any kind of combination, so that expression increases resistance to CSD disease.
1. A recombinant expression vector comprising a cDNA molecule comprising a nucleotide sequence encoding an amino acid sequence having amino acids 1974-2188 of SEQ ID NO: 2, operably linked to a heterologous promoter.
2. An isolated nucleic acid molecule of claim 1 , wherein said nucleotide sequence further encodes amino acids 1992-2188 of SEQ ID NO: 2.
3. A recombinant expression vector of claim 1 , wherein said nucleotide sequence further encodes amino acids 127-337 of SEQ ID NO: 2.
4. A recombinant expression vector of claim 1 , wherein said nucleotide sequence further encodes amino acids 897-1002 of SEQ ID NO: 2.
5. A recombinant expression vector of claim 1 , wherein said nucleotide sequence further encodes amino acids 1084-1315 of SEQ ID NO: 2.
6. A recombinant expression vector of claim 1 , wherein said nucleotide sequence further encodes amino acids 1-154 of SEQ ID NO: 3.
7. A recombinant expression vector of claim 1 , wherein said nucleotide sequence further encodes amino acids 1474-1890 of SEQ ID NO: 2.
8. A cDNA molecule comprising a nucleotide sequence consisting of nucleotides 6028-6675 of SEQ ID NO: 1, operably linked to a heterologous promoter.