Use of Linear Poly-Alpha-1,4-Glucans as Resistant Starch
The present invention concerns the use of water-insoluble linear poly-alpba-1,4-D-glucans as resistant starch (RS) as well as a process for the preparation of resistant starch characterised in that saccharose is reacted with a protein with the enzymatic activity of an amylosucrase.
1 . A method of preparing a resistant starch from water-insoluble linear poly-alpha-1,4-D-glucans that does not comprise one or more retrogradation steps.
2 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans is obtained by reacting an aqueous saccharose solution with an enzyme with the enzymatic activity of an amylosucrase.
3 . The method according to claim 2 , wherein the reaction of the aqueous saccharose solution is carried out with an enzyme having the enzymatic activity of an amylosucrase in vitro.
4 . The method according to claim 2 , wherein the reaction of the aqueous saccharose solution is carried out with an enzyme having the enzymatic activity of an amylosucrase in planta.
5 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans exhibit an RS content of more than 70 wt. %.
6 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans exhibit a DSC peak temperature of between 95° C. and 125° C.
7 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 1×10 2 g/mol to 10 5 g/mol.
8 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 1×10 3 g/mol to 3×10 4 g/mol.
9 . The method according to claim 1 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 2×10 3 g/mol to 1.2×10 4 g/mol.
10 . (canceled)
11 . The resistant starch produced by the method of claim 1 .
12 . The resistant starch of claim 11 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans exhibit an RS content of more than 70 wt. %.
13 . The resistant starch of claim 11 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans exhibit a DSC peak temperature of between 95° C. and 125° C.
14 . The resistant starch of claim 11 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 1×10 2 g/mol to 10 5 g/mol.
15 . The resistant starch of claim 11 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 1×10 3 g/mol to 3×10 4 g/mol.
16 . The resistant starch of claim 11 , wherein the water-insoluble linear poly-alpha-1,4-D-glucans have a mean molecular weight of 2×10 3 g/mol to 1.2×10 4 g/mol.
17 . A method for the preparation of resistant starch comprising:
a) preparing an aqueous saccharose solution:
b) converting the aqueous saccharose solution with a protein having the enzymatic properties of an amylosucrase into water-insoluble linear poly-alpha-1,4-D glucans; and optionally
c) isolating the water-insoluble linear poly-alpha-1,4-D-glucans.
18 . The method according to claim 17 , wherein the reaction of the aqueous saccharose solution is carried out with an enzyme having the enzymatic activity of an amylosucrase in vitro.
19 . The method according to claim 17 , wherein the reaction of the aqueous saccharose solution is carried out with an enzyme with the enzymatic activity of an amylosucrase in planta.