System for treating and preventing breast cancer
The present invention is directed to a system for treating individuals at risk of or suffering from breast cancer. The system comprises administering to the individual a recombinant poxvirus, where the poxvirus contains in a foreign nucleic acid encoding at least one breast cancer antigen.
1. A method for inducing an immunological response against a cell expressing a breast cancer associated antigen, wherein the breast cancer associated antigen is mucin and/or carcinoembryonic antigen, in a human, wherein the method comprises:
(a) selecting a human having breast cancer,
(b) administering to the individual a first poxvirus vector comprising SEQ ID NO:1 and SEQ ID NO:3,
(c) at regular intervals thereafter administering at least a second poxvirus vector comprising SEQ ID NO:1 and SEQ ID NO:3,
such that an immunological response against the cell expressing the breast cancer associated antigen is induced in the individual.
2. The method of claim 1 , further comprising administering at least one co-stimulatory molecule.
3. The method of claim 1 , further comprising administering granulocyte-macrophage colony stimulating factor (GM-CSF).
4. The method of claim 2 , wherein the co-stimulatory molecule is administered as a gene contained within the first, second, or both poxvirus vectors.
5. The method of claim 4 , wherein the first, second, or both poxvirus vectors contains at least B7.1, LFA-3 and ICAM-1 as co-stimulatory genes.
6. The method according to claim 1 , wherein the first and second poxvirus vectors are selected from the group consisting of: an orthopox virus vector; avipox virus vector; a suipox virus vector; a capripox virus vector; a leporipox virus vector; and an iridovirus vector, wherein the first and second poxvirus vectors can be the same or different poxvirus vectors.
7. The method according to claim 1 , wherein at least one of the poxvirus vectors is a replication impaired or non-replicating poxvirus vector.
8. The method according to claim 6 , wherein the first poxvirus vector is an orthopox vector.
9. The method according to claim 8 , wherein the orthopox virus vector is Wyeth vaccinia, MVA or NYVAC.
10. The method of claim 1 , wherein the first poxvirus vector is an orthopox vector, and the second poxvirus vector is an avipox vector.
11. The method of claim 10 , wherein the orthopox vector is vaccinia.
12. The method of claim 11 , wherein the vaccinia is vaccinia Wyeth or an attenuated vaccinia.
13. The method of claim 12 , wherein the attenuated vaccinia is MVA or NYVAC.
14. The method of claim 10 , wherein the orthopox vector is administered in one to three administrations at set intervals and the avipox vector is administered in multiple administrations at set intervals.
15. The method of claim 14 , wherein the set interval is 20 days to 90 days.
16. A method for inducing an immunological response against a cell expressing a breast cancer associated antigen, wherein the breast cancer associated antigen is mucin and/or carcinoembryonic antigen, in a human, wherein the method comprises:
(a) selecting a human having breast cancer,
(b) administering to the individual a first poxvirus vector comprising SEQ ID NO:1 and SEQ ID NO:3 and DNA sequences encoding ICAM-1, LFA-3, and B7.1, and
(c) at regular intervals thereafter administering at least a second poxvirus vector comprising SEQ ID NO:1 and SEQ ID NO:3 and DNA sequences encoding ICAM-1, LFA-3, and B7.1,
such that an immunological response against the cell expressing the breast cancer associated antigen is induced in the individual.
17. The method of claim 16 , further comprising administering granulocyte-macrophage colony stimulating factor (GM-CSF).
18. The method according to claim 16 , wherein the first and second poxvirus vectors are selected from the group consisting of: an orthopox virus vector; avipox virus vector; a suipox virus vector; a capripox virus vector; a leporipox virus vector; and an iridovirus vector, wherein the first and second poxvirus vectors can be the same or different poxvirus vectors.
19. The method according to claim 16 , wherein at least one of the poxvirus vectors is a replication impaired or non-replicating poxvirus vector.
20. The method according to claim 19 , wherein the first poxvirus vector is an orthopox vector.
21. The method according to claim 20 , wherein the orthopox virus vector is Wyeth vaccinia, MVA or NYVAC.
22. The method of claim 16 , wherein the first poxvirus vector is an orthopox vector, and the second poxvirus vector is an avipox vector.
23. The method of claim 22 , wherein the orthopox vector is vaccinia.
24. The method of claim 23 , wherein the vaccinia is vaccinia Wyeth or an attenuated vaccinia.
25. The method of claim 24 , wherein the attenuated vaccinia is MVA or NYVAC.
26. The method of claim 22 , wherein the orthopox vector is administered in one to three administrations at set intervals and the avipox vector is administered in multiple administrations at set intervals.
27. The method of claim 26 , wherein the set interval is 20 days to 90 days.