Methods of increasing lipolysis
Methods for increasing lipolysis in a cell are described herein. Such methods comprise the application of an electrical current to a cell. In an embodiment, the application of the electrical current does not substantially alter the viability of such cell and/or preserves the viability of such cell. In an embodiment, such cell is an adipocyte. Corresponding uses and packages are also described.
1. A method for increasing lipolysis in a fat cell of a subject, said method comprising applying an electrical current from about 2 mA to about 6 mA to said cell to depolarize the membrane of the cell, wherein said cell remains substantially viable following said application and lipolysis is increased in the depolarized cell.
2. The method of claim 1 , wherein said electrical current is biphasic.
3. The method of claim 1 , wherein said electrical current is monophasic.
4. The method of claim 1 , wherein said electrical current is about 4 mA.
5. The method of claim 1 , wherein said depolarization causes an alteration in the activity of an ion channel in said cell.
6. The method of claim 5 , wherein said depolarization causes a decrease in the activity of an ion channel in said cell.
7. The method of claim 6 , wherein said ion channel is one from an ion channel family selected from the group consisting of EAG, KCNQ, SK, slo, and Kv.
8. The method of claim 7 , wherein the ion channel is selected from the group consisting of eag, erg, elk, KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5, Kv1, Kv2, Kv3, Kv4, Kv5, Kv6, Kv7, Kv8, and Kv9.
9. The method of claim 6 , wherein said ion channel is a potassium channel
10. The method of claim 1 , wherein said cell is an adipocyte.
11. The method of claim 1 , wherein said subject is a mammal.
12. The method of claim 11 , wherein said mammal is a human.
13. The method of claim 10 , wherein said adipocyte is located in a subcutaneous tissue, a visceral adipose tissue or an intramuscular tissue.
14. The method of claim 13 , wherein said subcutaneous tissue is located in a region selected from the group consisting of arm, knee, calf, abdomen, thigh, buttock and hip.
15. The method of claim 1 , wherein said increase in lipolysis is at least 1.5 fold with respect to the level of lipolysis in a control cell.