Device for microfluid analyses
The invention relates to a device for microfluid analyses for a substrate with plane base surface and cover surface, wherein a chamber is integrated in the substrate for receiving liquid with at least two admissions and a semipermeable or permeable membrane is arranged in the chamber, wherein the chamber is subdivided by the membrane into two sectional chambers with at least one admission each.
1. Device for microfluid analyses with a substrate with plane base and cover surfaces, wherein
a chamber for receiving liquid comprising at least two admissions is integrated in the substrate, and
in the chamber a semipermeable or permeable membrane is arranged, the chamber being subdivided into two sectional chambers with at least one admission each by the membrane,
wherein the substrate comprises a covering element, in the base surface of which a recess for the chamber is provided, and
wherein the covering element comprises an intermediate plate in which an opening for the chamber is provided, and a cover plate which is provided for covering the opening on one side of the intermediate plate.
2. Device according to claim 1 , wherein the sectional chambers are at least partially arranged in parallel one to another.
3. Device according to claim 1 , wherein the sectional chambers are arranged in a plane in parallel or perpendicularly to the base surface of the substrate.
4. Device according to claim 1 , wherein the membrane is arranged at least partially in a plane in parallel or perpendicularly to the base surface of the substrate.
5. Device for microfluid analyses with a substrate with plane base and cover surfaces, wherein
a chamber for receiving liquid comprising at least two admissions is integrated in the substrate, and
in the chamber a semipermeable or permeable membrane is arranged, the chamber being subdivided into two sectional chambers with at least one admission each by the membrane,
wherein the substrate comprises a covering element, in the base surface of which a recess for the chamber is provided,
wherein the covering element is a cover plate, and
wherein the membrane is arranged between the cover plate and the intermediate plate.
6. Device according to claim 5 , wherein the sectional chambers are at least partially arranged in parallel one to another.
7. Device according to claim 5 , wherein the sectional chambers are arranged in a plane in parallel or perpendicularly to the base surface of the substrate.
8. Device according to claim 5 , wherein the membrane is arranged at least partially in a plane in parallel or perpendicularly to the base surface of the substrate.
9. Device for microfluid analyses with a substrate with plane base and cover surfaces, wherein
a chamber for receiving liquid comprising at least two admissions is integrated in the substrate, and
in the chamber a semipermeable or permeable membrane is arranged, the chamber being subdivided into two sectional chambers with at least one admission each by the membrane,
wherein the substrate comprises a covering element, in the base surface of which a recess for the chamber is provided, and
wherein the substrate comprises a cover element for covering the recess.
10. Device according to claim 9 , wherein the sectional chambers are at least partially arranged in parallel one to another.
11. Device according to claim 9 , wherein the sectional chambers are arranged in a plane in parallel or perpendicularly to the base surface of the substrate.
12. Device according to claim 9 , wherein the cover element is a plastic foil.
13. Device according to claim 12 , wherein the plastic foil comprises optically high-grade plastics.
14. Device according to claim 12 , wherein the plastic foil has a thickness of 50 μm 1 mm.
15. Device for microfluid analyses with a substrate with plane base and cover surfaces, wherein
a chamber for receiving liquid comprising at least two admissions is integrated in the substrate, and
in the chamber a semipermeable or permeable membrane is arranged, the chamber being subdivided into two sectional chambers with at least one admission each by the membrane,
wherein the substrate comprises a covering element, in the base surface of which a recess for the chamber is provided,
wherein the admissions end in the cover surface of the covering element of the substrate, and
wherein furthermore at least one liquid reservoir is provided which is arranged on the covering element of the substrate and in which an admission ends.
16. Device according to claim 15 , wherein the base and/or cover surface and/or membrane consists of an optically high-grade material which has autofluorescence as low as or lower than COC or COP.
17. Device according to claim 15 , wherein the sectional chambers are at least partially arranged in parallel one to another.
18. Device according to claim 15 , wherein the sectional chambers are arranged in a plane in parallel or perpendicularly to the base surface of the substrate.
19. Device according to claim 15 , wherein the membrane is arranged at least partially in a plane in parallel or perpendicularly to the base surface of the substrate.
20. Device according to claim 15 , wherein the chamber comprises at least four admissions, and the membrane subdivides the chamber into two sectional chambers with at least two admissions each.
21. Device according to claim 15 , wherein the membrane is flexible.
22. Device according to claim 21 , wherein the membrane is elastic.
23. Device according to claim 15 , wherein the membrane comprises at least one pore, each pore having a pore diameter in a predetermined partial area of the area of 1 nm-20 μm.
24. Device according to claim 23 , wherein each pore has a pore diameter of 0.5 μm-20 μm.
25. Device according to claim 15 , wherein the substrate comprise plastics.
26. Device according to claim 25 , wherein the plastics includes at least one of optically high-grade plastics and optically non-transparent plastics.
27. Device according to claim 15 , wherein the membrane and/or one chamber wall has a surface functionalization.
28. Device according to claim 27 , wherein the surface functionalization comprises a coating.
29. Device according to claim 28 , wherein the coating includes at least one of a polyelectrolyte film, an adhesion factor, a functional group, a biomolecule, a lipid membrane, a cell layer and a blocking molecule.
30. Device according to claim 15 , wherein the at least one liquid reservoir is of plastics.
31. Device according to claim 30 , wherein the at least one liquid reservoir is of the same plastics as the covering element in the area of the admission port.
32. Device according to claim 15 , wherein the liquid reservoir and the covering element are formed in one piece in the area of the admission port.
33. Device according to claim 32 , wherein the one piece is a molded part.