Alcohol dehydrogenases
The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to nonhuman hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohols which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohols with the formation of aldehydes or ketones.
1. An isolated polypeptide that has the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase and which comprises the sequence of SEQ ID NO:34.
2. The polypeptide of claim 1 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
3. A process for producing an organic product, comprising reacting the polypeptide of claim 1 with a substrate of said polypeptide and, optionally, with a cofactor of said polypeptide.
4. The process of claim 3 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
5. The process of claim 3 , further comprising:
a) isolating the product of the reaction;
b) processing the product to give a medicament.
6. The process of claim 5 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
7. The process of claim 3 , wherein said organic product is an enantiomerically pure alcohol.
8. The process of claim 7 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
9. A composition comprising the polypeptide of claim 1 and, in addition, at least one organic compound.
10. The composition of claim 9 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
11. The composition of claim 9 , wherein said organic compound is a carbonyl compound or an alcohol.
12. The composition of claim 11 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
13. The composition of claim 9 , further comprising NADH, NADPH, NAD + or NADP + .
14. The composition of claim 13 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
15. The composition of claim 13 , wherein said the organic compound is a carbonyl compound or an alcohol.
16. The composition of claim 15 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
17. The composition of claim 9 , wherein said organic compound is an aldehyde, a ketone, a primary alcohol or a chiral secondary alcohol.
18. The composition of claim 17 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.
19. The composition of claim 9 , wherein said organic compound is an asymmetrically substituted ketone.
20. The composition of claim 19 , wherein said polypeptide consists of the amino acid sequence of SEQ ID NO:34.