Glycopeptides derived from pancreatic structures, antibodies and applications thereof in diagnostics and therapeutics
View Patent ↗The invention relates to a glycopeptide comprising between 1 and 40 repeated C-terminal polypeptides, with 11 amino acids, of BSDL or FAPP, whereby the aforementioned polypeptides are glycosylated and bear glycosylated epitopes giving rise to a specific immunological reaction with induced antibodies in a patient suffering from type 1 diabetes, and/or purified from biological fluids of human or animal origin or recombinant and produced by expression in a standard host cell comprising an enzymatic material necessary for priming a glycosylation, said host cell being genetically modified such as to comprise a gene coding for the aforementioned polypeptides and a gene coding for one or more enzymes selected from among glycosyltransferases and anti-glycopeptide antibodies. The invention also relates to the applications thereof in therapeutics and diagnostics.
1. A monoclonal antibody selected from the group consisting of monoclonal antibody 16D10 or an antigen binding fragment thereof, having binding specificity for glycosylated bile salt dependent lipase (BSDL) or fetoacinar pancreatic protein (FAPP) and produced by the hybridoma deposited under the accession number I-3188; a monoclonal antibody, or an antigen binding fragment thereof, which binds to the same epitope of the BSDL or FAPP protein as monoclonal antibody 16D10; and a derivative of the 16D10 monoclonal antibody selected from the group consisting of a chimeric 16D10, humanized 16D10 or single chain scFv 16D10 antibody.
2. The monoclonal antibody according to claim 1 , wherein said derivative of the 16D10 monoclonal antibody is humanized 16D10 or chimeric 16D10.
3. The monoclonal antibody according to claim 1 , wherein the monoclonal antibody is of the IgG type.
4. The monoclonal antibody according to claim 1 , wherein the derivative of monoclonal antibody 16D10 is a single chain scFv 16D10 antibody.
5. A kit for diagnosis of a pancreatic pathology, comprising a monoclonal antibody according to claim 1 and a means for detecting the immunological complex resulting from the immunological reaction between the biological sample and said antibody.
6. A method of detection in vitro of a subject suffering from a pancreatic pathology, comprising contacting a biological sample from the subject with monoclonal antibody according to claim 1 and detecting the formation of immunological complexes resulting from the immunological reaction between said antibody and said biological sample.
7. The method according to claim 6 , wherein said biological sample is a sample of pancreatic tissue.
8. The method according to claim 6 , wherein said biological sample is a biological fluid selected from pancreatic juices, serum or urine.
9. The method according to claim 6 , wherein the method enables the detection of a subject suffering from pancreatic cancer.
10. The monoclonal antibody according to claim 2 , wherein said derivative of the 16D10 monoclonal antibody is humanized 16D10.
11. The monoclonal antibody according to claim 2 , wherein said derivative of the 16D10 monoclonal antibody is chimeric 16D10.
12. The monoclonal antibody according to claim 1 , wherein said monoclonal antibody is the 16D10 antibody, having binding specificity for glycosylated bile salt dependent lipase (BSDL) or fetoacinar pancreatic protein (FAPP) and produced by the hybridoma deposited under the accession number I-3188.
13. The monoclonal antibody according to claim 1 , wherein said monoclonal antibody is an antigen binding fragment of the 16D10 antibody.
14. The monoclonal antibody according to claim 1 , wherein said monoclonal antibody is a single chain scFv antibody.
15. The monoclonal antibody according to claim 1 , further comprising a label.
16. A composition comprising a pharmaceutically acceptable excipient and a labeled or unlabeled monoclonal antibody selected from the group consisting of: monoclonal antibody 16D10, or an antigen binding fragment thereof, having binding specificity for glycosylated bile salt dependent lipase (BSDL) or fetoacinar pancreatic protein (FAPP) and produced by the hybridoma deposited under the accession number I-3188; a monoclonal antibody, or an antigen binding fragment thereof, which binds to the same epitope of the BSDL or FAPP protein as monoclonal antibody 16D10; and a derivative of the 16D10 antibody selected from the group consisting of a chimeric 16D10, humanized 16D10 or single chain scFv 16D10 antibody.
17. The composition according to claim 16 , wherein said labeled or unlabeled derivative of the 16D10 monoclonal antibody is humanized 16D10 or chimeric 16D10.
18. The composition according to claim 16 , wherein the labeled or unlabeled monoclonal antibody is of the IgG type.
19. The composition according to claim 16 , wherein the labeled or unlabeled monoclonal antibody is a derivative of the 16D10 antibody that is a single chain scFv 16D10 antibody.
20. The composition according to claim 17 , wherein said labeled or unlabeled derivative of monoclonal antibody is 16D10 is humanized 16D10.
21. The composition according to claim 17 , wherein said labeled or unlabeled derivative or monoclonal antibody is 16D10 is chimeric 16D10.
22. The composition according to claim 16 , wherein said labeled or unlabeled monoclonal antibody is the 16D10 antibody, having binding specificity for glycosylated bile salt dependent lipase (BSDL) or human fetoacinar pancreatic protein (FAPP) and produced by the hybridoma deposited under the accession number I-3188.
23. The composition according to claim 16 , wherein said labeled or unlabeled monoclonal antibody is an antigen binding fragment of the 16D10 antibody.
24. The composition according to claim 16 , wherein said labeled or unlabeled monoclonal antibody is a single chain scFv antibody.
25. A hybridoma producing the monoclonal antibody is the 16D10 antibody, said hybridoma deposited under the accession number I-3188 and said antibody having binding specificity for glycosylated bile salt dependent lipase (BSDL) or fetoacinar pancreatic protein (FAPP).
26. The composition according to claim 16 , wherein said monoclonal antibody is a monoclonal antibody, or an antigen binding fragment thereof, which binds to the same epitope of the BSDL or FAPP protein as monoclonal antibody 16D10.
27. The monoclonal antibody according to claim 1 , wherein said monoclonal antibody is a monoclonal antibody, or an antigen binding fragment thereof, which binds to the same epitope of the BSDL or FAPP protein as monoclonal antibody 16D10.