Optical structures for metal-enhanced sensing
View Patent ↗The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.
1. A sensor using metal enhanced fluorescence, comprising:
a light emitting diode (LED) having a conical shaped depression on a front end surface;
said conical shaped depression having curved sides;
said curved sides having a conductive coating on an outer surface with respect to said LED; and
wherein a radius of curvature of said curved sides is set to provide directional emissions, and
wherein said directional emissions are induced by a surface plasmon excitation of a fluorophore disposed adjacent to said conductive coating.
2. A sensor according to claim 1 , wherein said conical shaped depression contains a solution containing analytes or antibodies to be analyzed.
3. A sensor according to claim 2 , further comprising:
an optical plug shaped to fit in said conical shaped depression;
a detector that detects fluorescence emissions;
a fiber having a first and second end; and
said fiber coupled to said optical plug at said first end and said detector at said second end.
4. A sensor according to claim 1 , further comprising:
a plasmon reflection signal detector; and
a fluorescence emissions detector; and
wherein said plasmon reflection signal detector is provided to detect said directional emissions and said fluorescence emissions detector is provided to detect fluorescence emissions.
5. A sensor according to claim 1 , wherein a plasmon reflection signal and a fluorescence emissions appear distinctly separated when viewed from the top of said LED.
6. A sensor according to claim 4 , wherein said plasmon reflection signal detector and said fluorescence emissions detector are located outside of said LED.
7. A sensor according to claim 4 , wherein said plasmon reflection signal detector and said fluorescence emissions detector are located inside of said LED.
8. A sensor according to claim 1 , wherein a reflective surface is used to reflect said directed emissions to a plasmon reflection signal detector and fluorescence emissions to a fluorescence emissions detector.
9. A sensor according to claim 1 , further comprising:
a porous silica layer on top of said conductive coating;
wherein said porous silica provides a size inclusion/exclusion sensing of different sized weakly fluorescent species.
10. A method of detection, comprising:
forming a front end surface of a light emitting diode (LED) to have a depression with said depression having curved sides;
setting a radius of curvature of said curved sides to provide directional emissions;
coating on an outer surface of said curved sides with a conductive material; and
inducing directional emission by surface plasmon excitation of a fluorophore disposed adjacent to said conductive material.