Method of making an anti-infective composition for treating oral infections
This invention concerns a method of making an anti-infective composition for oral administration using an agent selected from the group consisting of lactoferrin protein and lysozyme protein. The method comprises three steps. The nucleic acid, which encodes a lactoferrin or lysozyme protein, is expressed in a monocot seed. The protein is isolated from the seed. This protein isolation compromises greater than 3% of the total soluble protein in the monocot seed. The protein is then formulated for oral administration.
1. A method of making an anti-infective composition suitable for oral administration comprising an agent including at least one protein selected from the group consisting of lactoferrin protein and lysozyme protein, comprising the steps of:
a) expressing a nucleic acid encoding at least one protein selected from the group consisting of human lactoferrin protein and human lysozyme protein in a monocot seed;
b) isolating the at least one protein from the seed, wherein the at least one protein comprises about 3% or greater of the total soluble protein in the seed;
c) combining the at least one protein with a pharmaceutically acceptable carrier or diluent to provide a composition suitable for oral administration.
2. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the composition of the protein for oral administration is a lozenge.
3. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the composition of the protein for oral administration is a chewing gum.
4. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the composition of the protein for oral administration is a dissolving film.
5. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the composition of the protein for oral administration is a suspension.
6. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the composition of the protein for oral administration is a solution.
7. The method of making the anti-infective composition for oral administration according to claim 1 , further comprising administering the protein on epithelial surfaces of the oral cavity.
8. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the at least one protein comprises about >5% or greater of the total soluble protein in the seed.
9. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the at least one protein comprises about >10% or greater of the total soluble protein in the seed.
10. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the at least one protein comprises about >15% or greater of the total soluble protein in the seed.
11. The method of making the anti-infective composition for oral administration according to claim 1 , wherein the at least one protein comprises about >20% or greater of the total soluble protein in the seed.