Composition, method and use of bi-functional biomaterials
View Patent ↗The present invention includes a bifunctional specificity structure that includes a peptide linker having a first and a second binding domain, wherein the first binding domain is selective for a first biomaterial and the second binding domain is selective for a second biomaterial. The present invention also includes a method of making and identifying the bifunctional structure of the present invention and methods of using the same.
1. An engineered bifunctional peptide comprising:
a first peptide binding domain with length of about 7 amino acids to about 30 amino acids which binds selectively to a biomaterial wherein the first peptide binding domain is defined as a peptide amino acid sequence selected from the group consisting of the amino acid sequence of SEQ. ID. NOS.: 1-22;
a second peptide binding domain with length of about 7 amino acids to about 30 amino acids which binds selectively to an electrically conductive polymer comprising polypyrrole; and
a linker joining together the first and second peptide binding domains;
wherein selective binding means that the first and second peptide binding domains include a sequence from a peptide that is not removed during washing with Tris-buffered saline containing 0.1% (vol/vol) Tween-20 (0.1% TBS-T) while bound to the biomaterial or electrically conductive polymer.
2. The engineered bifunctional peptides recited in claim 1 , wherein the biomaterial is a biodegradable material.
3. The engineered bifunctional peptides recited in claim 1 , wherein the first peptide binding domain is defined further as a peptide which binds non-covalently.
4. The engineered bifunctional peptides recited in claim 1 , wherein the biomaterial is selected from the group consisting of a nucleic acid, peptide, and protein.