Method for distinguishing ulcerative colitis from Crohn's disease by detecting the presence of fecal anit-neutrophil cytoplasmic antibodies (ANCA)
A method and apparatus for the differentiation of ulcerative colitis from Crohn's disease and other gastrointestinal illnesses using the presence of anti-neutrophil cytoplasmic antibodies (ANCA) as a marker of ulcerative colitis is described. The apparatus consists of either a qualitative enzyme-linked immunoassay or other immunoassay that utilizes antibodies specific to human immunoglobulins for the measurement of total endogenous ANCA in a human sample. The method and apparatus can be used by healthcare providers to distinguish ulcerative colitis from Crohn's disease and other gastrointestinal illnesses.
1. A method for diagnosing ulcerative colitis by testing a fecal sample for an elevated level of anti-neutrophil cytoplasmic antibodies, the method comprising:
obtaining a fecal sample from a person presenting with inflammatory bowel disease;
determining whether there is an elevated level of anti-neutrophil cytoplasmic antibodies in the sample compared to an anti-neutrophil cytoplasmic antibody level in a healthy sample, wherein an elevated level of anti-neutrophil cytoplasmic antibodies is indicative of ulcerative colitis; and
diagnosing the person with anti-neutrophil cytoplasmic antibodies present in the fecal sample with ulcerative colitis.
2. The method as recited in claim 1 , further comprising:
diluting the fecal sample.
3. The method as recited in claim 2 , further comprising:
contacting the fecal sample with neutrophil cytoplasmic antigens to create a treated sample.
4. The method as recited in claim 3 , further comprising:
contacting the treated sample with polyvalent antibodies to human immunoglobulin to create a readable sample.
5. The method as recited in claim 4 , further comprising:
determining an optical density of the readable sample at 450 nm, wherein the optical density corresponds to a level of anti-neutrophil cytoplasmic antibodies in the sample.
6. A diagnostic assay for diagnosing ulcerative colitis by determining whether a fecal sample contains an elevated level of anti-neutrophil cytoplasmic antibodies, the assay comprising:
obtaining a human fecal sample from a person presenting with inflammatory bowel disease;
diluting the fecal sample;
contacting the diluted sample with neutrophil cytoplasmic antigens to create a treated sample;
contacting the treated sample with polyvalent antibodies to human immunoglobulin to create a readable sample;
determining the optical density of the readable sample at 450 nm; and
determining whether the optical density indicates an elevated level of anti-neutrophil cytoplasmic antibodies compared to an anti-neutrophil cytoplasmic antibody level in a healthy sample, where an elevated level of anti-neutrophil cytoplasmic antibodies is an indicator of ulcerative colitis.
7. The diagnostic assay as recited in claim 6 , wherein the anti-neutrophil cytoplasmic antibodies are one of IgG, IgE, IgM, IgD, IgA sec , IgA, and combinations thereof.
8. The diagnostic assay as recited in claim 6 , wherein the assay is selected from a group consisting of an enzyme-linked immunoassay and a lateral flow membrane test.