Screening methods for identifying compounds which decrease HIV entry into a cell
A peptide has an amino acid sequence having more than 80% homology with the amino acid sequence listed as SEQ ID NO:4. A nucleic acid molecule has more than 80% homology with one of the nucleic acid sequences listed as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. Ligands, anti-ligands, cells vectors relating to the peptide and/or nucleic acid molecule are also used.
1. A method for identifying a compound which decreases infectivity of a cell by HIV comprising:
(a) contacting a cell which expresses a polypeptide comprising the sequence of SEQ ID NO: 5 with a candidate compound which binds to said polypeptide;
(b) contacting said cell with HIV; and
(c) measuring infectivity of said cell by said HIV, wherein if infectivity is decreased then said candidate compound is identified as a compound which decreased infectivity of a cell by HIV.
2. The method of claim 1 , wherein HIV infectivity is decreased by at least two-fold.
3. The method according to claim 1 , wherein said infectivity of the cell by HIV is measured by measuring the production of an HIV protein.
4. The method according to claim 3 , wherein said HIV protein is p24.
5. A method for identifying a compound which decreases infectivity of a cell by HIV comprising:
(a) contacting a polypeptide of SEQ ID NO:5 with a candidate compound and detecting binding of said candidate compound to said polypeptide, wherein if said candidate compound binds to said polypeptide, then;
(b) contacting a cell which expresses a polypeptide comprising the sequence of SEQ ID NO: 5 with said candidate compound of step (a) which binds to said polypeptide;
(c) contacting said cell with HIV; and
(d) measuring infectivity of said cell by said HIV, wherein if infectivity is decreased then said candidate compound is identified as a compound which decreases infectivity of a cell by HIV.
6. The method of claim 5 , wherein HIV infectivity is decreased by at least two-fold.
7. The method according to claim 5 , wherein said infectivity of the cell by HIV is measured by measuring the production of an HIV protein.
8. The method according to claim 7 , wherein said HIV protein is p24.
9. A method for identifying a compound which decreases entry of HIV into a cell comprising:
(a) contacting a polypeptide of SEQ ID NO:5 with a candidate compound and detecting binding of said candidate compound to said polypeptide, wherein if said candidate compound binds to said polypeptide, then;
(b) contacting a cell which expresses a polypeptide comprising the sequence of SEQ ID NO: 5 with said candidate compound of step (a) which binds to said polypeptide;
(c) contacting said cell with HIV; and
(d) measuring the entry of said HIV into said cell, wherein if entry is decreased then said candidate compound is identified as a compound which decreases the entry of HIV into a cell.
10. The method of claim 9 , wherein HIV entry is decreased by at least two-fold.
11. The method according to claim 9 , wherein said entry of HIV into a cell is measured by measuring the production of an HIV protein.
12. The method according to claim 11 , wherein said HIV protein is p24.