Region of factor IXa protease domain that interacts with factor VIIIa and methods therefor
Novel polypeptides or derivatives comprising the factor VIIIa binding site on factor IXa are disclosed. The novel polypeptides or derivatives have anti-coagulation activity. Nucleic acids encoding those polypeptides are also disclosed. Methods for identifying an agent having anti-coagulation activity are also disclosed. These methods comprise determining whether the agent displaces the polypeptide or derivative from its factor VIIIa binding site. The agent identified in these methods is also useful in methods for treating a patient to prevent thrombosis. The treatment methods comprise administration of the agent to the patient. Additional methods are also disclosed for treating a patient to prevent thrombosis, comprising treating the patient with a polypeptide or derivative comprising the factor VIIIa binding site on factor IXa. Methods of preventing coagulation in a blood sample are also disclosed, comprising adding the polypeptides or derivatives described above to the blood sample. Methods of detecting factor VIIIa in a sample are also disclosed. Those methods comprise contacting the sample with the above-described polypeptide or derivative, wherein the polypeptide or derivative also comprises a covalently attached detectable moiety, then determining whether the polypeptide or derivative is binding factor VIIIa from the sample.
1. A polypeptide of from 6 to 20 amino acids capable of inhibiting the activation of factor X in the presence of coagulation factor VIIIa, said polypeptide comprising SEQ ID NO:5 (LVX 1 X 2 AT), wherein X 1 is tyrosine and X 2 is either arginine or glutamine or wherein X 1 is either aspartic acid or tyrosine and X 2 is glutamine.
2. The polypeptide of claim 1 wherein the polypeptide comprises, SEQ ID NO:7 or SEQ ID NO:8.
3. A polypeptide of from 9 to 20 amino acids capable of inhibiting the activation of factor X in the presence of coagulation factor VIIIa, said polypeptide comprising SEQ ID NO:9, provided that Xaa of SEQ ID NO:9 is not cysteine.
4. The polypeptide of claim 3 wherein the polypeptide comprises SEQ ID NO:10.
5. A composition having anticoagulation activity wherein the composition comprises a polypeptide of from 6 to 20 amino acids capable of inhibiting the activation of factor X in the presence of coagulation factor VIIIa, said polypeptide comprising SEQ ID NO:5 (LVX 1 X 2 AT), wherein X 1 is tyrosine and X 2 is either arginine or glutamine or wherein X 1 is either aspartic acid or tyrosine and X 2 is glutamine.
6. The composition of claim 5 wherein the polypeptide comprises SEQ ID NO:7.
7. The composition of claim 5 wherein the polypeptide comprises SEQ ID NO:8.
8. A composition having anticoagulation activity wherein the composition comprises a polypeptide of from 9 to 20 amino acids capable of inhibiting the activation of factor X in the presence of coagulation factor VIIIa, said polypeptide comprising SEQ ID NO:9, provided that Xaa of SEQ ID NO:9 is not cysteine.
9. The composition of claim 8 wherein the polypeptide is SEQ ID NO:10.
10. The polypeptide of claim 1 , wherein said polypeptide further comprises a covalently attached detectable moiety.
11. The polypeptide of claim 10 , wherein said detectable moiety is radioactive or fluorescent.
12. The polypeptide of claim 2 , wherein said polypeptide further comprises a covalently attached detectable moiety.
13. The polypeptide of claim 12 , wherein said detectable moiety is radioactive or fluorescent.
14. The polypeptide of claim 3 , wherein said polypeptide further comprises a covalently attached detectable moiety.
15. The polypeptide of claim 14 , wherein said detectable moiety is radioactive or fluorescent.
16. The polypeptide of claim 4 , wherein said polypeptide further comprises a covalently attached detectable moiety.
17. The polypeptide of claim 16 , wherein said detectable moiety is radioactive or fluorescent.
18. A six amino acid polypeptide capable of inhibiting the activation of factor X in the presence of coagulation factor VIII, said six amino acid polypeptide comprising SEQ ID NO:5 (LVX 1 X 2 AT), wherein X 1 is either aspartic acid or tyrosine and X 2 is either arginine or glutamine.
19. The polypeptide of claim 18 , wherein said polypeptide further comprises a covalently attached detectable moiety.
20. The polypeptide of claim 19 , wherein said detectable moiety is radioactive or fluorescent.
21. The six amino acid polypeptide of claim 18 , wherein said six amino acid polypeptide comprises SEQ ID NO: 6 SEQ ID NO:7 or SEQ ID NO:8.
22. A six amino acid pojypeptide of capable of inhibiting the activation of factor X in the presence of coagulation factor VIII, said six amino acid polypeptide consisting of SEQ ID NO:6 (LVDRAT).