Modulators of polysaccharides and uses thereof
The present invention provides peptides with a specific affinity for glycosaminoglycan molecules. These peptides may have any number of functions, including but not limited to use as inhibitors of glycosaminoglycan-mediated processes, enhancers of glycosaminoglycan-mediated processes, and as molecular probes to identify the presence of a specific glycosaminoglycan. Peptides of the invention may be directed against any glycosaminoglycan, including hyaluronic acid, chondroitin sulfate A, chondroitin sulfate C, dermatan sulfate, heparin, keratan sulfate, keratosulfate, chitin, chitosan 1, and chitosan 2. These isolated peptides may have therapeutic uses in the treatment or prevention of diseases involving infection, inflammatory diseases, cancer, infections, etc. The peptides may also have other biological functions such as contraception.
1. A method for inhibiting an immune reaction, said method comprising administering a peptide consisting essentially of the amino acid sequence of:
i) (Gly-Ala-His-Trp-Gln-Phe-Asu-Ala-Leu-Thr-Val-Arg (SEQ ID NO:1) or a conservative amino acid substitution thereof at a residue position other than 4,5,6,9, 10 or 11;
ii) Gly-Ala-Ala-Trp-Gln-Phe-Asn-Ala-Leu-Thr-Val-Arg (SEQ ID NO:7) or a conservative amino acid substitution thereof at a residue position other than 4,5,6,9, 10 or 11;
iii) Gly-Ala-His-Trp-Gln-Phe-Ala-Ala-Leu-Thr-Val-Arg (SEQ ID NO:8) or a conservative amino acid substitution thereof at a residue position other than 4,5,6,9, 10 or 11; and
iv) Gly-Ala-His-Trp-Gln-Phe-Asn-Ala-Leu-Thr-Val-Ala (SEQ ID NO:9) or a conservative amino acid substitution thereof at a residue position other than 4,5,6,9, 10 or 11,
wherein the peptide selectively binds hyaluronic acid that inhibits an immune reaction.
2. The method of claim 1 , wherein the immune reaction is cutaneous.
3. The method of claim 1 , wherein the peptide inhibits leukocyte infiltration.
4. The method of claim 1 , wherein said method comprises administering:
a carrier material; and
a said peptide to a subject with an immune reaction.