Crystallization of fibroblast activation protein alpha (FAPα)
Provided are crystals relating to FAPα and its various uses.
1. A composition comprising a protein in crystalline form wherein the protein consists of SEQ ID NO:3, and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=70.3 Å b=152.60 Å and c=214.65 Å.
2. A composition according to claim 1 wherein the protein is present in the protein crystal as a dimer.
3. A composition according to claim 1 wherein the protein crystal diffracts X-rays for a determination of structure coordinates to a resolution less than 3.0 Angstroms.
4. A method of obtaining a crystal of SEQ ID NO: 3 comprising:
Forming a crystallization volume comprising a precipitate solution and a protein that consists of SEQ ID NO:3, and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=70.3 Å, b=152.60 Å, and c=214.65 Å; and storing the crystallization volume under conditions suitable for crystal formation until a crystal is formed.
5. A method according to claim 4 wherein the protein is present in the protein crystal as a dimer.
6. A method according to claim 4 wherein the protein crystal diffracts X-rays for a determination of structure coordinates to a resolution less than 3.0 Angstroms.
7. A non-crystalline protein consisting of SEQ ID NO:3.
8. A non-crystalline protein consisting of residues 27-760 of SEQ ID NO:1.
9. The protein according to claim 8 wherein the protein is expressed from a nucleic acid molecule that consists of SEQ ID NO:2.
10. An isolated non-crystalline protein consisting of residues 27-760 of SEQ ID NO:1.
11. An isolated noncrystalline protein consisting of SEQ ID NO:3.
12. A method of obtaining the three-dimensional structure of the protein of SEQ ID NO: 3:
(a) Crystallize a protein consisting of SEQ ID NO: 3 to obtain a protein crystal having a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=70.3 Å, b=152.60 Å, and c=214.65 Å;
(b) Use the crystal of (a) to obtain an X-ray diffraction pattern; and
(c) Solve the three-dimensional structure of the protein from the diffraction pattern, and thereby obtain the three-dimensional structure.