Host/vector system for expression of membrane proteins
A method of expressing proteins is disclosed. In a preferable embodiment, the method comprises placing a DNA sequence encoding a protein or peptide and expression vector containing a regulatable promoter expressible in Rhodospirillum rubrum and expressing the protein within a bacterial host, wherein the host has extra capacity for membrane formation and wherein the host is a member of the genus Rhodospirillum.
1. A method of expressing proteins comprising the steps of
(a) placing a DNA sequence encoding a protein or peptide in an expression vector comprising a regulatable promoter expressible in Rhodospirillum rubrum , wherein the DNA sequence encoding the protein or peptide is operably connected to the promoter, and
(b) expressing the protein within a bacterial host, wherein the host has extra capacity for membrane formation and wherein the host is a member of the genus Rhodospirillum.
2. The method of claim 1 wherein the protein or peptide is a membrane protein or peptide.
3. The method of claim 1 wherein the protein or peptide is a heterologous protein or peptide.
4. The method of claim 1 wherein the host is Rhodospirillum rubrum P5 or Rhodospirillum rubrum P4.
5. The method of claim 1 wherein the expression vector comprises a promoter that can be induced by reduction of oxygen tension.
6. A protein expression system comprising:
a vector comprising a DNA molecule encoding a protein or peptide in an expression vector comprising a regulatable promoter expressible in R. rubrum , wherein the vector is contained within a host of the genus Rhodospirillum and wherein the host has extra capacity for membrane formation and wherein the DNA molecule encoding the protein or peptide is operably connected to the promoter.
7. The protein expression system of claim 6 wherein the protein or peptide is a heterologous protein or peptide.
8. The protein expression system of claim 6 , wherein the protein or peptide is a membrane protein or peptide.