GB virus C and methods of treating viral infections
The present application provides GB virus C sequences (GBV-C or hepatitis G virus) and methods of using the sequences.
1. An isolated and purified nucleic acid molecule encoding an infectious GBV-C, wherein the nucleic acid molecule encodes SEQ ID NO:20, or a variant thereof, wherein said variant is 99% identical to SEQ ID NO:20.
2. The isolated and purified nucleic acid molecule of claim 1 , wherein the nucleic acid molecule encodes SEQ ID NO:20.
3. The isolated and purified nucleic acid molecule of claim 1 , wherein the nucleic acid is RNA.
4. The isolated and purified nucleic acid molecule of claim 1 , wherein the nucleic acid is DNA.
5. The nucleic acid molecule of claim 1 , further comprising a heterologous nucleic acid sequence.
6. The nucleic acid molecule of claim 5 , wherein the heterologous nucleic acid sequence encodes a polypeptide.
7. The nucleic acid molecule of claim 6 , wherein the polypeptide is a mammalian polypeptide.
8. The nucleic acid molecule of claim 1 , further comprising a heterologous promoter.
9. A host cell comprising a nucleic acid molecule encoding an infectious GBV-C, wherein the nucleic acid molecule encodes SEQ ID NO:20, or a variant thereof, wherein said variant is 99% identical to SEQ ID NO:20.
10. The host cell of claim 9 comprising a nucleic acid molecule that encodes SEQ ID NO:20.
11. The host cell of claim 10 , wherein the cell is a mammalian cell.
12. The host cell of claim 11 , wherein the cell is a lymphocyte cell.
13. The host cell of claim 12 , wherein the cell is CD4+ lymphocyte cell.
14. An isolated and purified nucleic acid molecule comprising SEQ ID NO:19.