Adeno-associated virus serotype 1 nucleic acid sequences, vectors and host cells containing same
View Patent ↗The nucleic acid sequences of adeno-associated virus (AAV) serotype 1 are provided, as are vectors and host cells containing these sequences and functional fragments thereof. Also provided are methods of delivering genes via AAV-1 derived vectors.
1. A recombinant vector comprising a 5′ AAV-1 inverted terminal repeat (ITR) and a heterologous nucleic acid for delivery to a cell, wherein said ITR has the sequence consisting of:
nt 1 to 143 of SEQ ID NO: 1.
2. The recombinant vector according to claim 1 , wherein said vector further comprises a 3′ AAV-1 ITR.
3. The recombinant vector according to claim 1 , wherein said vector further comprises AAV-1 capsid proteins having the sequence of SEQ ID NO: 13, 15 or 17.
4. The recombinant vector according to claim 1 , wherein said vector further comprises adenovirus sequences.
5. A recombinant vector comprising a 3′ AAV-1 inverted terminal repeat (ITR) and a heterologous nucleic acid for delivery to a cell, wherein said ITR has the sequence of:
nt 4576 to 4718 of SEQ ID NO: 1.
6. The recombinant vector according to claim 5 , wherein said vector further comprises a 5′ AAV-1 ITR.
7. An isolated host cell transduced with the recombinant vector according to claim 3 .
8. A composition comprising a recombinant virus having an AAV-1 capsid comprising an AAV-1 protein selected from among AAV-1 vp1 having the amino acid sequence of SEQ ID No: 13; AAV-1 vp2 having the amino acid sequence of SEQ ID NO: 15 and AAV-1 vp3 having the amino acid sequence of SEQ ID NO: 17 and a heterologous molecule which comprises an AAV 5′ inverted terminal repeat sequence (ITR), a transgene, and an AAV 3′ ITR.
9. The composition of claim 8 wherein the AAV-1 protein vp1 is encoded by a nucleic acid having at least 98% identity to nucleotides 2223–4431 of SEQ ID NO: 1.
10. The composition of claim 8 wherein the AAV-1 protein vp2 is encoded by a nucleic acid having at least 98% identity to nucleotides 2634–4432 of SEQ ID NO: 1.
11. The composition of claim 8 wherein the AAV-1 protein vp3 is encoded by a nucleic acid having at least about 98% identity to nucleotides 2829–4432 of SEQ ID NO: 1.
12. The composition of claim 8 wherein the AAV 5′ ITR and 3′ ITR are of AAV serotype 2.
13. The composition of claim 12 wherein the recombinant virus further comprises a regulatable promoter which directs expression of the transgene.