Cells and improved method for preserving cells
A method for maintaining a viability level of a metabolite in a biological sample comprising disposing a biological sample into a solute solution comprising a solute and a chemical selected from the group consisting of a monosaccharide, a monosaccharide polyol, a cell metabolite-controlling agent, a salt, and mixtures thereof; and incubating the biological sample in the solute solution while maintaining a viability level of a metabolite in the biological sample.
1 . A method for loading a biological sample with a solute comprising:
disposing a biological sample into a solute solution comprising a solute and a chemical selected from the group consisting of a monosaccharide, a monosaccharide polyol, a cell metabolite-controlling agent, a salt, and mixtures thereof; and
incubating the biological sample in the solute solution while maintaining a positive solute concentration increase (mM) to incubation (hours) loading gradient during incubating to load the biological sample with the solute.
2 . The method of claim 1 wherein said incubating comprises incubating the biological sample at a temperature ranging from about 35° C. to about 39° C. and from about 6 hours to about 10 hours.
3 . The method of claim 1 wherein said monosaccharide polyol comprises mannitol.
4 . The method of claim 1 wherein said cell metabolite-controlling agent comprises adenine.
5 . The method of claim 1 wherein said monosaccharide comprises glucose.
6 . The method of claim 1 wherein said metabolite comprises ATP.
7 . The method of claim 1 wherein said biological sample comprises a mammalian biological sample.
8 . The method of claim 1 wherein said solute solution additionally comprises a buffering salt compound.
9 . A biological sample produced in accordance with the method of claim 1 .
10 . A method for maintaining a viability level of a metabolite in a biological sample comprising:
disposing a biological sample into a solute solution comprising a solute and a chemical selected from the group consisting of a monosaccharide, a monosaccharide polyol, a cell metabolite-controlling agent, a salt, and mixtures thereof; and
incubating the biological sample in the solute solution while maintaining a viability level of a metabolite in the biological sample.
11 . The method of claim 12 additionally comprising maintaining a positive gradient of metabolite level during said incubating.
12 . The method of claim 10 wherein said maintaining a viability level of a metabolite comprises preventing a decrease in a metabolite level of the biological sample.
13 . The method of claim 10 wherein said metabolite is selected from the group consisting of ATP, 2,3-DPG and mixtures thereof.
14 . The method of claim 10 wherein said incubating the biological sample in the solute solution comprises incubating at a temperature ranging from about 35° C. to about 39° C. for a period of time ranging from about 4 hours to about 8 hours.
15 . A method for maintaining a viability level of a metabolite in a biological sample comprising:
disposing a biological sample into a solute solution comprising a solute and a chemical selected from the group consisting of a monosaccharide, a monosaccharide polyol, a cell metabolite-controlling agent, a salt, and mixtures thereof; and
incubating the biological sample in the solute solution while maintaining a maintaining a positive gradient of change in a level of a metabolite to change in duration of incubation.
16 . The method of claim 15 wherein said metabolite comprises ATP.