Salicylic acid-binding protein encoding nucleic acid, SABP2, and methods of use thereof
View Patent ↗Novel nucleic acid molecules encoding SA-binding proteins involved in SA-mediated disease resistance responses are disclosed. Methods of use of the nucleic acid molecules and proteins of the invention are also provided.
1. An isolated SABP2 nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) the nucleotide sequence of SEQ ID NO: 2; and
b) a nucleotide sequence encoding the polypeptide of SEQ ID NO: 2.
2. The isolated nucleic acid molecule of claim 1 , wherein said SABP2 nucleic acid molecule comprises SEQ ID NO: 1.
3. The isolated nucleic acid molecule of claim 1 , wherein said SABP2 nucleic acid molecule comprises a NUCLEOTIDE sequence encoding SEQ ID NO: 2.
4. The isolated nucleic acid molecule of claim 1 , which is a DNA molecule.
5. An RNA molecule encoded by the SABP2 nucleic acid molecule of claim 1 .
6. An expression vector comprising the nucleic acid molecule of claim 1 .
7. The expression vector of claim 6 wherein said vector is selected from the group of vectors consisting of plasmid, cosmid, baculovirus, bacteria, yeast and viral vectors.
8. A host cell transformed with the expression vector of claim 6 .
9. The host cell of claim 8 , wherein said host cell is selected from the group consisting of a plant cell bacterial cell, yeast cell, and insect cell.
10. A method for enhancing resistance of a plant to a plant pathogen comprising transforming the plant with the nucleic acid molecule of claim 1 .
11. The method of claim 10 , wherein said nucleic acid molecule comprises SEQ ID NO: 1.
12. The method of claim 10 , wherein the nucleic acid molecule encodes SEQ ID NO: 2.
13. A transgenic plant comprising the ISOLATED nucleic acid molecule of claim 1 .
14. The transgenic plant of claim 13 , wherein said plant is TOBACCO.
15. The transgenic plant of claim 13 , wherein said plant has increased resistance to disease.
16. The transgenic plant of claim 13 wherein said nucleic acid molecule comprises SEQ ID NO: 1.
17. The transgenic plant of claim 13 , wherein the nucleic acid molecule encodes SEQ ID NO: 2.
18. The method of claim 10 , wherein said nucleic acid molecule is overexpressed in said plant, and wherein said overexpression enhances the resistance of said plant to a plant pathogen.
19. The host cell of claim 8 , wherein said plant cell is from a plant selected from the group consisting of tobacco, Arabidopsis , rice, maize, wheat, soybean, tomato, potato, barley, and canola.
20. The method of claim 10 , further comprising contacting said plant or a plant cell obtained therefrom with a pathogen responsive agent, and assaying SABP2 function in the presence or absence of said agent in said plant, plant cell or extracts thereof.