Ancestral and COT viral sequences, proteins and immunogenic compositions
The present invention is directed to ancestral and COT nucleic acid and amino acid sequences, methods for producing such sequences and uses thereof, including prophylactic and diagnostic uses.
1. An isolated expression construct comprising the following operably linked elements: a transcriptional promoter; a nucleic acid sequence that encodes the polypeptide encoded by SEQ ID NO:25; and a transcriptional terminator.
2. The expression construct of claim 1 , wherein the transcriptional promoter is a heterologous promoter.
3. The expression construct of claim 2 , wherein the promoter is a cytomegalovirus promoter.
4. An isolated prokaryotic or eukaryotic cell transformed or transfected with the expression construct of claim 1 .
5. The eukaryotic cell of claim 4 , which is a mammalian cell.
6. The prokaryotic cell of claim 4 , which is an E. coli cell.
7. The eukaryotic cell of claim 4 , which is an S. cerevisiae cell.
8. The eukaryotic cell of claim 4 , which is a human cell.
9. A vector comprising the expression construct of claim 1 .
10. The vector of claim 9 , wherein the nucleic acid sequence is operably linked to a Semlike Forest Virus replicon, and wherein the resulting recombinant replicon is operably linked to a cytomegalovirus promoter.
11. An isolated host cell comprising the vector of claim 9 .