Method of cancer treatment by p53 protein control
Method of cancer treatment by controlling cellular p53 protein levels. The invention concerns, in particular, the use of a compound capable of modulating calpaine activity.
1. A method for detecting an inhibitor of p53 protein degradation comprising providing a cell extract containing one or more p53 protein and one or more proteases, administering a peptide or protein inhibitor of calpain protease activity of to the cell extract, and measuring p53 protein and p53 protein fragments, wherein the inhibitor administered is a calpastatin.
2. The method of claim 1 , wherein the calpastatin is encoded by one of SEQ ID NO: 1-3.
3. The method of claim 1 , wherein the cell extract is derived from a tumor cell.
4. The method of claim 1 , wherein the inhibitor administered is a fragment of calpastatin.
5. The method of claim 3 , wherein the inhibitor is a fragment of calpastatin.
6. The method of claim 1 , wherein measuring the p53 protein and p53 protein fragments is performed using gel electrophoresis.