Crystallization of 11-Beta-Hydroxysteroid Dehydrogenase type 1
Provided are crystals relating to 11-Beta-Hydroxysteroid Dehydrogenase type 1 and its various uses.
1. A composition comprising a protein in crystalline form wherein the protein consists of SEQ ID No: 5 and wherein the protein crystal has a crystal lattice in a P2 1 space group and unit cell dimensions, +/− 5% of a=56.4 Å, b=152.5 Å, c=73.8 Å, α=90°, β=92.2° and γ=90°.
2. A composition according to claim 1 wherein the protein crystal diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
3. A method for forming a crystal of a protein comprising:
forming a crystallization volume comprising a precipitant solution and a protein that consists of SEQ ID No: 5 and wherein the protein crystal has a crystal lattice in a P2 1 space group and unit cell dimensions, +/− 5% of a=56.4 Å, b=152.5 Å, c=73.8 Å, α=90°, β=92.2° and γ=90°; and storing the crystallization volume under conditions suitable for crystal formation of the protein.
4. A method according to claim 3 wherein the protein diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
5. A method according to claim 3 , the method further comprising diffracting the protein crystal to produce a diffraction pattern and solving the structure of the protein from the diffraction pattern.
6. A soluble protein consisting of SEQ ID No: 5.
7. A non-crystalline protein consisting of SEQ ID No: 5.
8. An isolated soluble protein consisting of SEQ ID No: 5.
9. An isolated non-crystalline protein consisting of SEQ ID No: 5.