Promoting cardiac cell differentiation
Compositions and methods for inducing a cardiac muscle phenotype, such as rhythmic contraction and formation of myofibrils, in a cell are described. Disclosed are nucleic acids encoding myofibrillogenesis-inducing RNA (MIR) molecules, RNA molecules (MIR) and RNA-binding proteins that bind to MIR to induce myofibrillogenesis and vectors comprising these nucleic acids. Also included is a method of promoting cardiac muscle differentiation using these compositions.
1. A purified nucleic acid comprising nucleotide sequence SEQ ID NOS: 1 or 5, wherein said sequences are capable of being transcribed into a myofibrillogenesis-inducing RNA (MIR) molecule.
2. The purified nucleic acid of claim 1 , wherein said nucleotide sequence is transcribed into an RNA molecule having a secondary structure that permits specific binding to at least one MIR-binding protein.
3. A purified myofibrillogenesis-inducing RNA (MIR) molecule comprising a ribonucleic acid sequence which is fully complementary over the full length of SEQ ID NO: 5 and induces myofibrillogenesis in living cells.
4. A vector comprising a purified nucleic acid cDNA of SEQ ID NO: 5 that is transcribed into a myofibrillogenesis-inducing RNA (MIR) molecule.
5. The vector of claim 4 , wherein the purified nucleic acid further encodes a MIR-binding protein.