Mammalian sphingosine kinase type 2 isoforms, cloning, expression and methods of use thereof
View Patent ↗Methods for detecting agents or drugs which inhibit or promote an activity of sphingosine kinase type 2 isoform involving providing a recombinant DNA construct into a cell such that sphingosine kinase type 2 isoform is produced in the cell, adding at least one drug or agent to the cell, and detecting whether or not the drug or agent inhibits or promotes an activity of sphingosine kinase type 2 isoform by measuring sphingosine kinase-depedendent phosphorylation of lipids in the cell and comparing the resultant measurement to a control which did not receive the drug or agent, wherein a decrease in the amount of sphingosine kinase-dependent phosphorylation of the lipids as compared to the control indicates an inhibitory drug or agent, or an increase in the amount of sphingosine kinase-dependent phosphorylation of the lipids in the cell as compared to the control indicates a stimulatory drug or agent.
1. A method for detecting an agent or a drug which inhibits or promotes an enzymatic activity of a sphingosine kinase type 2 isoform encoded by (a) a DNA having a nucleotide sequence consisting essentially of the nucleotide sequence of SEQ ID NO: 11 or (b) a DNA encoding protein consisting essentially of the amino acid sequence of SEQ ID NO: 12, the method comprising:
(i) providing a vector comprising said DNA and a cell comprising said vector such that said sphingosine kinase type 2 isoform is produced in said cell,
(ii) adding at least one drug or agent to said cell, and
(iii) detecting whether or not said drug or agent inhibits or promotes the enzymatic activity of the sphingosine kinase type 2 isoform by measuring an amount of lipids phosphorylated by said sphingosine kinase type 2 isoform in said cell and comparing the resultant measurement to a control which did not receive said drug or agent, wherein a decrease in the amount of lipids phosphorylated by said sphingosine kinase type 2 isoform as compared to the control indicates an inhibitory drug or agent, or an increase in the amount of lipids phosphorylated by said sphingosine kinase type 2 isoform in said cell as compared to the control indicates a stimulatory drug or agent.
2. The method according to claim 1 , wherein said lipids comprise D,L-threo-dihydrosphingosine.
3. The method according to claim 1 , wherein said lipids comprise phytosphingosine.