Crystallization of the catalytic domain of human phosphodiesterase 4-D3 (PDE4-D3)

1. A composition comprising a protein-Rolipram complex in co-crystalline form, wherein the protein of the complex consists of residues 24-357 of SEQ ID NO:3, wherein said protein is in complex with Rolipram, and wherein the co-crystal has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=110.48 Å, b=110.47 Å, c=166.20 Å, and α=β=γ=90°.

2. The composition according to claim 1 wherein the co-crystal diffracts X-rays for a determination of structure coordinates to a resolution having a value of 3.0 Å or less.

3. A method comprising:

forming a co-crystallization volume comprising a precipitant solution, Rolipram, and a protein consisting of residues 24-357 of SEQ ID NO:3;

storing the crystallization volume under conditions suitable for co-crystal formation of Rolipram and the protein; and

forming a protein-Rolipram co-crystal, wherein said protein is in complex with Rolipram, and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=110.48 Å, b=110.47 Å, c=166.20 Å, and α=β=γ=90°.

4. The method according to claim 3 wherein the protein-Rolipram co-crystal is formed that diffracts X-rays for a determination of structure coordinates to a resolution having a value of 3.0 Å or less.

5. The method according to claim 3 wherein the protein-Roliplram co-crystal is formed that diffracts X-rays for a determination of structure coordinates to a resolution having a value of 2.5 Å or less.

6. A non-crystalline protein consisting of residues 24-357 of SEQ ID NO:3.

7. A non-crystalline protein consisting of SEQ ID NO:3.

8. An isolated non-crystalline protein consisting of residues 24-357 of SEQ ID NO: 3.

9. An isolated non-crystalline protein consisting of SEQ ID NO: 3.