Methods and articles for the delivery of therapeutic agents
Articles and methods for the delivery of drugs and/or nucleic acids. Articles including a nanoparticle are provided that may be used for the delivery of a drug, a nucleic acid, or both, to a subject. The articles may be of polymeric material and may self-assemble.
1. An article for delivering a drug and a nucleic acid, the article comprising:
a nanoparticle micelle, wherein the nanoparticle comprises a polymer having a polyester backbone comprising N-methyldietheneamine sebacate and [(cholesteryl oxocarbonylamido ethyl) methyl bis (ethylene) ammonium bromide] sebacate;
a nucleic acid associated with an exterior of the micelle; and
a drug associated with an interior of the micelle.
2. The article of claim 1 , wherein the nanoparticle is capable of passing through a cell membrane.
3. The article of claim 1 , wherein the nucleic acid is DNA.
4. The article of claim 1 , wherein the drug is a cancer drug.
5. The article of claim 1 , wherein the nanoparticle is stable at a concentration of greater than 5 mg/L.
6. The article of claim 1 , wherein the nanoparticle is capable of crossing the blood/brain barrier.
7. A composition comprising the article of claim 1 and a pharmaceutically acceptable carrier.
8. The article of claim 1 , wherein the polyester backbone comprises tertiary amines.
9. The article of claim 8 , wherein the polyester backbone comprises a copolymer of quaternized and non-quaternized tertiary ammonium groups.
10. The article of claim 1 , wherein the polyester backbone further comprises an ether linkage.
11. The article of claim 8 , wherein at least a portion of the tertiary amines are quaternized and bound to a hydrophobic side chain.
12. The article of claim 1 , wherein the polyester backbone further comprises a polyether.
13. A kit comprising:
a container including an amphoteric polymeric nanoparticle micelle, wherein the polymeric nanoparticle comprises a polymer having a polyester backbone comprising N-methyldietheneamine sebacate and [(cholesteryl oxocarbonylamido ethyl) methyl bis (ethylene) ammonium bromide] sebacate;
a nucleic acid associated with an exterior of the micelle;
a drug associated with an interior of the micelle; and
instructions for administering the nanoparticle to a subject.
14. The kit of claim 13 , wherein the polyester backbone comprises a graft co-polymer.
15. The article of claim 1 , wherein the polyester backbone comprises an amphiphilic co-polymer.
16. The article of claim 15 , wherein the amphiphilic co-polymer is a cationic amphiphilic co-polymer.
17. The article of claim 1 , wherein the drug is not covalently or ionically bound to the nanoparticle.
18. The article of claim 1 , wherein the drug is physically contained by the nanoparticle.
19. The article of claim 1 , wherein the polyester backbone comprises a graft co-polymer.
20. The article of claim 11 , wherein the hydrophobic side chain comprises cholesterol.