Solid salts of benzazepine compounds and pharmaceutical compositions containing them
Pharmaceutically acceptable metal salts of compounds of formula (I): in which R 1 is selected from the group consisting of (C 1 –C 6 )alkoxy(C 1 –C 6 )alkyl which may be substituted with (C 1 –C 6 )alkoxy, phenyl-(C 1 –C 6 )-alkyl and/or phenyloxy-(C 1 –C 6 )-alkyl group in which any phenyl group may be substituted with (C 1 –C 6 )alkyl, (C 1 –C 6 )alkoxy or halogen, and naphtyl-(C 1 –C 6 )-alkyl; R 2 and R 3 are each independently hydrogen or halogen; R 4 is a biolabile ester forming group; and in which the salt is selected from the lithium salt and salts of bivalent metal ions such as magnesium, calcium and zinc. Also described are a method for the preparation of the above salts; pharmaceutical compositions comprising the above salts; methods of using the above salts to treat heart disorders or hypertension, to improve gastrointestinal blood flow, or to treat or inhibit cardiac damage induced by adriamycin and comparable anti-cancer drugs; and crystalline S-α-methylbenzylamine salts of the compounds of formula (I) that are useful as intermediates in production of the above salts.
1. A pharmaceutically acceptable salt selected from the group consisting of the lithium, calcium, magnesium and zinc salts of 3-[[[1-[(2R) -2-(ethoxycarbonyl)-4-phenylbutyl]cyclopentyl]carbonyl]amino]-2,3,4,5-tetrahydro-2-oxo-(3S)-1H-1-benzazepine-1-acetic acid, and the calcium salt of 3-[[[1-[2-(ethoxycarbonyl)-4-(1-naphthalenyl)butyl]cyclopentyl]carbonyl]amino]-2,3,4,5-tetrahydro-oxo-,[S-(R*,S*)]-1H-1H-1-benzazepine-1-acetic acid.
2. A salt according to claim 1 , wherein said salt is a calcium salt.
3. A method of preparing a salt
according to claim 1 ; said method comprising mixing a solution or slurry of the hydroxide of lithium, calcium, magnesium or zinc with a solution or slurry of 3-[[[1-[(2R)-2-(ethoxycarbonyl)-4-phenylbutyl]cyclopentyl]carbonyl]-amino]-2,3,4,5-tetrahydro-2-oxo-(3S)-1H-1-benzazepine-1-acetic acid, or a solution or slurry of calcium hydroxide with a solution or slurry of 3-[[[1-[2-(ethoxycarbonyl)-4-(1-naphthalenyl)butyl]cyclopentyl]carbonyl]amino]-2,3,4,5-tetrahydro-oxo-,[S-(R*,S*)]-1H-1H-1-benzazepine-1-acetic acid, in a slightly polar aprotic solvent to yield a homogeneous solution of the salt in said slightly polar aprotic solvent.
4. A method according to claim 3 , wherein said slightly polar aprotic solvent is methyl tertiary-butyl ether or ethyl acetate.
5. A method according to claim 3 , further comprising isolating said salt by mixing the solution of the salt in the slightly polar aprotic solvent with a precipitant to crystallize or precipitate the salt in solid form.
6. A method according to claim 5 , wherein the mixing with the precipitant is preceded by azeotropic removal of water.
7. A method according to claim 5 , wherein said precipitant is a linear (C 4 –C 10 )-hydrocarbon.
8. A method according to claim 7 , wherein said precipitant is n-hexane.
9. A pharmaceutical composition comprising at least one salt according to claim 1 and a pharmaceutical carrier or adjuvant.
10. A composition according to claim 9 , wherein said composition is in a form suitable for IV administration.
11. A method of treating a heart disorder in a patient, said method comprising administering to a patient in need thereof an effective heart disorder treating amount of a salt according to claim 1 .
12. A method of improving gastrointestinal blood flow in a patient, said method comprising administering to a patient in need thereof an effective gastrointestinal blood flow improving amount of a salt according to claim 1 .
13. A method of treating hypertension, said method comprising administering to a patient in need thereof an effective hypertension treating amount of a salt according to claim 1 .
14. A method of treating or inhibiting cardiac damage induced by a cardiac damage inducing anti-cancer drug, said method comprising administering to a patient treated with said anti-cancer drug an effective cardiac damage inhibiting amount of a compound according to claim 1 .
15. A method according to claim 14 , wherein said anti-cancer drug is adriamycin.