Method for differentiation of Alzheimer's Disease into subgroups
View Patent ↗A method for diagnosing distinct subgroups of Alzheimer's Disease, the method comprising the steps of obtaining a sample of cerebrospinal fluid and determining the level of ubiquitin, the level of Aβ 1-42 , and the level of tau present in the sample. Based on the levels of each composition in the cerebrospinal fluid, the sample can be assigned to distinct subgroups.
1. A method for diagnosing a distinct subgroup of Alzheimer's Disease, the method comprising the steps:
(1) obtaining a sample of cerebrospinal fluid;
(2) determining whether the level of ubiquitin is equal to or greater than 500 ng/ml wherein a level equal to or greater than 500 ng/ml assigns said sample to a first subgroup, if not then;
(3) determining whether the level of Aβ 1-42 is equal to or greater than 900 pg/ml and if so assigning to a second subgroup, if not then;
(4) determining whether the level of tau is equal to or greater than 920 pg/ml and if so, assigning said sample to a third subgroup, if not then;
(5) determining whether the level of tau is equal to or greater than 520 pg/ml and if so, assigning said sample to a fourth subgroup, if not then assigning said sample to a fifth subgroup.
2. The method of claim 1 , wherein the step of determining the level of ubiquitin includes assaying by competitive ELISA using a monoclonal antibody which recognizes the amino acid residues 64-76 of ubiquitin.
3. The method of claim 1 , wherein the level of tau is determined by sandwich ELISA.
4. The method of claim 1 wherein the levels of Aβ 1-42 are determined by sandwich ELISA.