IP Library Patent Application 10892854
Patent Application
App. No. 10/892,854

Amplification

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Quick Facts
Patent No.
US None
App. No.
10/892,854
Abstract

Primer mixtures and methods of investigating single nucleotide polymorphisms in samples of DNA are provided having particular primer sequences. The primer mixtures and methods are aimed at achieving optimised performance in multiplexes.

Claims (969)

1 . A mixture including at least one primer pair, at least one of those primer pairs being a primer pair having or including one of the following sequences:

Pair 1

Primer 1

cgacgtggtggatgtgctagccag

SEQ ID NO 1

atgtttctcaagtggtcctg

Primer 2

tgacgtggctgacctgagacaaag

SEQ ID NO 2

tggtttctcaagtggtccca

Pair 2

Primer 1

cgacgtggtggatgtgctagcctc

SEQ ID NO 4

tgctt- taaccacaccagca

Primer 2

tgacgtggctgacctgagaccctc

SEQ ID NO 5

tgcttt- aaccacaccagcc

Pair 3

Primer 1

cgacgtggtggatgtgctagacca

SEQ ID NO 7

accccacaaagcagg

Primer 2

tgacgtggctgacctgagacacca

SEQ ID NO 8

accccacaaagcagc

Pair 4

Primer 1

cgacgtggtggatgtgctagctct

SEQ ID NO 10

ttccacctgaaagcaaggg

Primer 2

tgacgtggctgacctgagacctct

SEQ ID NO 11

ttccacctgaaagcaaggt

Pair 5

Primer 1

cgacgtggtggatgtgctagcgca

SEQ ID NO 13

gcccttgccatctg

Primer 2

tgacgtggctgacctgagaccgca

SEQ ID NO 14

gcccttgccatctc

Pair 6

Primer 1

cgacgtggtggatgtgctagctta

SEQ ID NO 16

gatgtcacctcttc- catgcac

Primer 2

tgacgtggctgacctgagacctta

SEQ ID NO 17

gatgtcacctct- tccatgcag

Pair 7

Primer 1

cgacgtggtggatgtgctaggcgg

SEQ ID NO 19

gaggaaggaagggagg

Primer 2

tgacgtggctgacctgagacgcgg

SEQ ID NO 20

gaggaaggaagggagc

Pair 8

Primer 1

cgacgtggtggatgtgctaggatg

SEQ ID NO 22

cctcttgcattgtgaacg

Primer 2

tgacgtggctgacctgagacgatg

SEQ ID NO 23

cctcttgcattgtgaacc

Pair 9

Primer 1

cgacgtggtggatgtgctagcatt

SEQ ID NO 25

gtgtttcaaacgcgtgcc

Primer 2

tgacgtggctgacctgagaccatt

SEQ ID NO 26

gtgtttcaaacgcgtgct

Pair 10

Primer 1

cgacgtggtggatgtgctagtgcc

SEQ ID NO 28

act- ctgacactgatgcttg

Primer 2

tgacgtggctgacctgagactgcc

SEQ ID NO 29

act- ctgacactgatgcttc

Pair 11

Primer 1

cgacgtggtggatgtgctagctgc

SEQ ID NO 31

cttggctcccagcc

Primer 2

tgacgtggctgacctgagacctgc

SEQ ID NO 32

cttggctcccagct

Pair 12

Primer 1

cgacgtggtggatgtgctagcaca

SEQ ID NO 34

aagctgatctgagcacctattac

Primer 2

tgacgtggctgacctgagaccaca

SEQ ID NO 35

aagctgatctgagcacctattag

Pair 13

Primer 1

cgacgtggtggatgtgctaggctg

SEQ ID NO 37

tgggctctat- gtatcatccaac

Primer 2

tgacgtggctgacctgagacgctgt

SEQ ID NO 38

gggctc- tatgtatcatccaag

Pair 14

Primer 1

cgacgtggtggatgtgctaggccc

SEQ ID NO 40

aatggctaatttccttacatg

Primer 2

tgacgtggctgacctgagacgccc

SEQ ID NO 41

aatggctaatttccttacatc

Pair 15

Primer 1

cgacgtggtggatgtgctagggga

SEQ ID NO 43

aactgctgggtctgt

Primer 2

tgacgtggctgacctgagacggga

SEQ ID NO 44

aactgctgggtctgc

Pair 16

Primer 1

cgacgtggtggatgtgctagctgt

SEQ ID NO 46

gcatccactgcgcc

Primer 2

tgacgtggctgacctgagacctgt

SEQ ID NO 47

gcatccactgcgca

Pair 17

Primer 1

cgacgtggtggatgtgctagcctg

SEQ ID NO 49

gagcatgagc- tgaccac

Primer 2

tgacgtggctgacctgagaccctg

SEQ ID NO 50

gagcatgagctg- accaa

Pair 18

Primer 1

cgacgtggtggatgtgctagccat

SEQ ID NO 52

gcctcacctcctgcatt

Primer 2

tgacgtggctgacctgagacccat

SEQ ID NO 53

gcctcacctcctgcatc

Pair 19

Primer 1

cgacgtggtggatgtgctaggcat

SEQ ID NO 55

gccattgccaaattcc

Primer 2

tgacgtggctgacctgagacgcat

SEQ ID NO 56

gccattgccaaattct

Pair 20

Primer 1

cgacgtggtggatgtgctaggcca

SEQ ID NO 58

accagacctcccagg

Primer 2

tgacgtggctgacctgagacgcca

SEQ ID NO 59

accagacctcccagc

Pair 21

Primer 1

cgacgtggtggatgtgctagggga

SEQ ID NO 61

gacaggcccatgc- a

Primer 2

tgacgtggctgacctgagacggga

SEQ ID NO 62

gacaggcccatgct

Pair 22

Primer 1

cgacgtggtggatgtgctagcaga

SEQ ID NO 64

aaaggca- ggaacctggaca

Primer 2

tgacgtggctgacctgagaccaga

SEQ ID NO 65

aaaggca- ggaacctggact

Pair 23

Primer 1

cgacgtggtggatgtgctaggagc

SEQ ID NO 67

caagaatcgcagggaa

Primer 2

tgacgtggctgacctgagacgagc

SEQ ID NO 68

caagaatcgcagggat

Pair 24

Primer 1

cgacgtggtggatgtgctagtgtg

SEQ ID NO 70

catgttccctggtgttca

Primer 2

tgacgtggctgacctgagactgtg

SEQ ID NO 71

catgttccctggtgttct

Pair 25

Primer 1

cgacgtggtggatgtgctagggca

SEQ ID NO 73

tacccatgtgactaaa- tgttga

Primer 2

tgacgtggctgacctgagacggca

SEQ ID NO 74

tacccatgtgact- aaatgttgt

Pair 26

Primer 1

cgacgtggtggatgtgctaggcaa

SEQ ID NO 76

ggcccaaagcaaagaa

Primer 2

tgacgtggctgacctgagacgcaa

SEQ ID NO 77

ggcccaaagcaaagat

Pair 27

Primer 1

cgacgtggtggatgtgctagagga

SEQ ID NO 79

cagtggcttctgtactgcta

Primer 2

tgacgtggctgacctgagacagga

SEQ ID NO 80

cagtggcttctgtactgctt

Pair 28

Primer 1

cgacgtggtggatgtgctagctgg

SEQ ID NO 82

aagggctttg- tttgccaa

Primer 2

tgacgtggctgacctgagacctgg

SEQ ID NO 83

aagggctttgt- ttgccat

Pair 29

Primer 1

cgacgtggtggatgtgctaggggg

SEQ ID NO 85

gtactggggagaccaa

Primer 2

tgacgtggctgacctgagacgggg

SEQ ID NO 86

gtactggggagaccat

or sequences at least 75% homologous thereto.

2 . A mixture according to claim 1 which includes at least 8 primer pairs having one or both of the sequences listed for the pairs of claim 1 .

3 . A mixture according to claim 1 which includes at least 8 primer pairs, from the listed primer pairs of claim 1 .

4 . A mixture according to claim 1 in which the mixture includes at least one primer pair, at least one of those primer pairs being a primer pair having or including one of the sequences of a primer pair selected from pair 1, pair 2, pair 3, pair 7, pair 8, pair 9, pair 10, pair 11, pair 12, pair 13, pair 14, pair 15, pair 16, pair 17, pair 18, pair 19, pair 20, pair 21, pair 22, pair 23, pair 24, pair 25, pair 26, pair 27, pair 28, pair 29 as provided in claim 1 .

5 . A mixture according to claim 1 in which the primers of a pair have a locus specific portion and a universal portion.

6 . A mixture according to claim 1 in which one or more of the listed sequences of the primers have locus specific portions and/or universal portions according to the following forms:

Pair 1

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 1

ccagatgtttctcaagtggtcctg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 2

aaagtggtttctcaagtggtccca

(locus specific portion)

Pair 2

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 4

cctctgctttaaccacaccagca

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 5

cctctgctttaaccacaccagcc

(locus specific portion)

Pair 3

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 7

accaaccccacaaagcagg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 8

accaaccccacaaagcagc

(locus specific portion)

Pair 4

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 10

ctctttccacctgaaagcaaggg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 11

ctctttccacctgaaagcaaggt

(locus specific portion)

Pair 5

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 13

cgcagcccttgccatctg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 14

cgcagcccttgccatctc

(locus specific portion)

Pair 6

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 16

cttagatgtcacctcttccatgcac

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 17

cttagatgtcacctcttccatgcag

(locus specific portion)

Pair 7

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 19

gcgggaggaaggaagggagg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 20

gcgggaggaaggaagggagc

(locus specific portion)

Pair 8

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 22

gatgcctcttgcattgtgaacg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 23

gatgcctcttgcattgtgaacc

(locus specific portion)

Pair 9

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 25

cattgtgtttcaaacgcgtgcc

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 26

cattgtgtttcaaacgcgtgct

(locus specific portion)

Pair 10

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 28

tgccactctgacactgatgcttg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 29

tgccactctgacactgatgcttc

(locus specific portion)

Pair 11

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 31

ctgccttggctcccagcc

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 32

ctgccttggctcccagct

(locus specific portion)

Pair 12

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 34

cacaaagctgatctgagcacctattac

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 35

cacaaagctgatctgagcacctattag

(locus specific portion)

Pair 13

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 37

gctgtgggctctatgtatcatccaac

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 38

gctgtgggctctatgtatcatccaag

(locus specific portion)

Pair 14

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 40

gcccaatggctaatttccttacatg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 41

gcccaatggctaatttccttacatc

(locus specific portion)

Pair 15

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 43

gggaaactgctgggtctgt

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 44

gggaaactgctgggtctgc

(locus specific portion)

Pair 16

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 46

ctgtgcatccactgcgcc

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 47

ctgtgcatccactgcgca

(locus specific portion)

Pair 17

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 49

cctggagcatgagctgaccac

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 50

cctggagcatgagctgaccaa

(locus specific portion)

Pair 18

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 52

ccatgcctcacctcctgcatt

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 53

ccatgcctcacctcctgcatc

(locus specific portion)

Pair 19

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 55

gcatgccattgccaaattcc

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 56

gcatgccattgccaaattct

(locus specific portion)

Pair 20

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 58

gccaaccagacctcccagg

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 59

gccaaccagacctcccagc

(locus specific portion)

Pair 21

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 61

gggagacaggcccatgca

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 62

gggagacaggcccatgct

(locus specific portion)

Pair 22

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 64

cagaaaaggcaggaacctggaca

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 65

cagaaaaggcaggaacctggact

(locus specific portion)

Pair 23

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 67

gagccaagaatcgcagggaa

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 68

gagccaagaatcgcagggat

(locus specific portion)

Pair 24

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 70

tgtgcatgttccctggtgttca

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 71

tgtgcatgttccctggtgttct

(locus specific portion)

Pair 25

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 73

ggcatacccatgtgactaaatgttga

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 74

ggcatacccatgtgactaaatgttgt

(locus specific portion)

Pair 26

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 76

gcaaggcccaaagcaaagaa

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 77

gcaaggcccaaagcaaagat

(locus specific portion)

Pair 27

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 79

aggacagtggcttctgtactgcta

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 80

aggacagtggcttctgtactgctt

(locus specific portion)

Pair 28

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 82

ctggaagggctttgtttgccaa

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 83

ctggaagggctttgtttgccat

(locus specific portion)

Pair 29

Primer 1

cgacgtggtggatgtgctag

(universal portion)

{close oversize brace}

SEQ ID NO 85

gggggtactggggagaccaa

(locus specific portion)

Primer 2

tgacgtggctgacctgagac

(universal portion)

{close oversize brace}

SEQ ID NO 86

gggggtactggggagaccat

(locus specific portion)

or sequences at least 75% homologous thereto in respect of the universal portion.

7 . A mixture according to claim 1 in which the mixture includes at least 5 primer pairs having or including one of the sequences of a primer pair selected from pair 1, pair 2, pair 3, pair 7, pair 8, pair 9, pair 10, pair 11, pair 12, pair 13, pair 14, pair 15, pair 16, pair 17, pair 18, pair 19, pair 20, pair 21, pair 22, pair 23, pair 24, pair 25, pair 26, pair 27, pair 28, pair 29 as provided in claim 1 .

8 . A mixture according to claim 1 in which the mixture includes at least 8 primer pairs having or including one of the sequences of a primer pair selected from pair 1, pair 2, pair 3, pair 7, pair 8, pair 9, pair 10, pair 11, pair 12, pair 13, pair 14, pair 15, pair 16, pair 17, pair 18, pair 19, pair 20, pair 21, pair 22, pair 23, pair 24, pair 25, pair 26, pair 27, pair 28, pair 29 as provided in claim 1 .

9 . A method of investigating single nucleotide polymorphisms in a sample of DNA, the method comprising contacting the DNA containing sample with at least one set of first primers, amplifying the DNA using those primers to give an amplified product, contacting at least a portion of the amplified product with at least one second set of primers, amplifying the DNA using those second set of primers to give a further amplified product and examining one or more characteristics of the further amplified product, in which the first primers include one or more pairs of primers pairs, at least one of those primer pairs being a primer pair having or including the following sequences:

Pair 1

Primer 1

cgacgtggtggatgtgctagccag

SEQ ID NO 1

atgtttctcaagtggtcctg

Primer 2

tgacgtggctgacctgagacaaag

SEQ ID NO 2

tggtttctcaagtggtccca

Pair 2

Primer 1

cgacgtggtggatgtgctagcctc

SEQ ID NO 4

tgctt- taaccacaccagca

Primer 2

tgacgtggctgacctgagaccctc

SEQ ID NO 5

tgcttt- aaccacaccagcc

Pair 3

Primer 1

cgacgtggtggatgtgctagacca

SEQ ID NO 7

accccacaaagcagg

Primer 2

tgacgtggctgacctgagacacca

SEQ ID NO 8

accccacaaagcagc

Pair 4

Primer 1

cgacgtggtggatgtgctagctct

SEQ ID NO 10

ttccacctgaaagcaaggg

Primer 2

tgacgtggctgacctgagacctct

SEQ ID NO 11

ttccacctgaaagcaaggt

Pair 5

Primer 1

cgacgtggtggatgtgctagcgca

SEQ ID NO 13

gcccttgccatctg

Primer 2

tgacgtggctgacctgagaccgca

SEQ ID NO 14

gcccttgccatctc

Pair 6

Primer 1

cgacgtggtggatgtgctagctta

SEQ ID NO 16

gatgtcacctcttc- catgcac

Primer 2

tgacgtggctgacctgagacctta

SEQ ID NO 17

gatgtcacctct- tccatgcag

Pair 7

Primer 1

cgacgtggtggatgtgctaggcgg

SEQ ID NO 19

gaggaaggaagggagg

Primer 2

tgacgtggctgacctgagacgcgg

SEQ ID NO 20

gaggaaggaagggagc

Pair 8

Primer 1

cgacgtggtggatgtgctaggatg

SEQ ID NO 22

cctcttgcattgtgaacg

Primer 2

tgacgtggctgacctgagacgatg

SEQ ID NO 23

cctcttgcattgtgaacc

Pair 9

Primer 1

cgacgtggtggatgtgctagcatt

SEQ ID NO 25

gtgtttcaaacgcgtgcc

Primer 2

tgacgtggctgacctgagaccatt

SEQ ID NO 26

gtgtttcaaacgcgtgct

Pair 10

Primer 1

cgacgtggtggatgtgctagtgcc

SEQ ID NO 28

act- ctgacactgatgcttg

Primer 2

tgacgtggctgacctgagactgcc

SEQ ID NO 29

act- ctgacactgatgcttc

Pair 11

Primer 1

cgacgtggtggatgtgctagctgc

SEQ ID NO 31

cttggctcccagcc

Primer 2

tgacgtggctgacctgagacctgc

SEQ ID NO 32

cttggctcccagct

Pair 12

Primer 1

cgacgtggtggatgtgctagcaca

SEQ ID NO 34

aagctgatctgagcacctattac

Primer 2

tgacgtggctgacctgagaccaca

SEQ ID NO 35

aagctgatctgagcacctattag

Pair 13

Primer 1

cgacgtggtggatgtgctaggctg

SEQ ID NO 37

tgggctctat- gtatcatccaac

Primer 2

tgacgtggctgacctgagacgctgt

SEQ ID NO 38

gggctc- tatgtatcatccaag

Pair 14

Primer 1

cgacgtggtggatgtgctaggccc

SEQ ID NO 40

aatggctaatttccttacatg

Primer 2

tgacgtggctgacctgagacgccc

SEQ ID NO 41

aatggctaatttccttacatc

Pair 15

Primer 1

cgacgtggtggatgtgctagggga

SEQ ID NO 43

aactgctgggtctgt

Primer 2

tgacgtggctgacctgagacggga

SEQ ID NO 44

aactgctgggtctgc

Pair 16

Primer 1

cgacgtggtggatgtgctagctgt

SEQ ID NO 46

gcatccactgcgcc

Primer 2

tgacgtggctgacctgagacctgt

SEQ ID NO 47

gcatccactgcgca

Pair 17

Primer 1

cgacgtggtggatgtgctagcctg

SEQ ID NO 49

gagcatgagc- tgaccac

Primer 2

tgacgtggctgacctgagaccctg

SEQ ID NO 50

gagcatgagctg- accaa

Pair 18

Primer 1

cgacgtggtggatgtgctagccat

SEQ ID NO 52

gcctcacctcctgcatt

Primer 2

tgacgtggctgacctgagacccat

SEQ ID NO 53

gcctcacctcctgcatc

Pair 19

Primer 1

cgacgtggtggatgtgctaggcat

SEQ ID NO 55

gccattgccaaattcc

Primer 2

tgacgtggctgacctgagacgcat

SEQ ID NO 56

gccattgccaaattct

Pair 20

Primer 1

cgacgtggtggatgtgctaggcca

SEQ ID NO 58

accagacctcccagg

Primer 2

tgacgtggctgacctgagacgcca

SEQ ID NO 59

accagacctcccagc

Pair 21

Primer 1

cgacgtggtggatgtgctagggga

SEQ ID NO 61

gacaggcccatgc- a

Primer 2

tgacgtggctgacctgagacggga

SEQ ID NO 62

gacaggcccatgct

Pair 22

Primer 1

cgacgtggtggatgtgctagcaga

SEQ ID NO 64

aaaggca- ggaacctggaca

Primer 2

tgacgtggctgacctgagaccaga

SEQ ID NO 65

aaaggca- ggaacctggact

Pair 23

Primer 1

cgacgtggtggatgtgctaggagc

SEQ ID NO 67

caagaatcgcagggaa

Primer 2

tgacgtggctgacctgagacgagc

SEQ ID NO 68

caagaatcgcagggat

Pair 24

Primer 1

cgacgtggtggatgtgctagtgtg

SEQ ID NO 70

catgttccctggtgttca

Primer 2

tgacgtggctgacctgagactgtg

SEQ ID NO 71

catgttccctggtgttct

Pair 25

Primer 1

cgacgtggtggatgtgctagggca

SEQ ID NO 73

tacccatgtgactaaa- tgttga

Primer 2

tgacgtggctgacctgagacggca

SEQ ID NO 74

tacccatgtgact- aaatgttgt

Pair 26

Primer 1

cgacgtggtggatgtgctaggcaa

SEQ ID NO 76

ggcccaaagcaaagaa

Primer 2

tgacgtggctgacctgagacgcaa

SEQ ID NO 77

ggcccaaagcaaagat

Pair 27

Primer 1

cgacgtggtggatgtgctagagga

SEQ ID NO 79

cagtggcttctgtactgcta

Primer 2

tgacgtggctgacctgagacagga

SEQ ID NO 80

cagtggcttctgtactgctt

Pair 28

Primer 1

cgacgtggtggatgtgctagctgg

SEQ ID NO 82

aagggctttg- tttgccaa

Primer 2

tgacgtggctgacctgagacctgg

SEQ ID NO 83

aagggctttgt- ttgccat

Pair 29

Primer 1

cgacgtggtggatgtgctaggggg

SEQ ID NO 85

gtactggggagaccaa

Primer 2

tgacgtggctgacctgagacgggg

SEQ ID NO 86

gtactggggagaccat

or sequences 75% homologous therewith.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 24, 2006
From: SECRETARY OF STATE FOR THE HOME DEPARTMENT, THE
To: FORENSIC SCIENCE SERVICE LTD.
Reel/Frame 017921/0073 →