The invention provides methods of obtaining immunogenic proteins from genomic sequences including Neisseria , including the amino acid sequences and the corresponding nucleotide sequences, as well as the genomic sequence of Neisseria meningitidis B . The proteins so obtained are useful antigens for vaccines, immunogenic compositions, and/or diagnostics.
1. An isolated and purified polynucleotide comprising the nucleotide of SEQ ID NO 35.
2. An isolated and purified polynucleotide comprising a nucleotide sequence having greater than 70% sequence identity to SEQ ID NO 35.
3. An isolated and purified polynucleotide which encodes a protein, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO 35 or a complement thereof.
4. An isolated and purified polynucleotide which encodes a protein, wherein the polynucleotide comprises a nucleotide sequence having greater than 70% sequence identity to SEQ ID NO 35 or a complement thereof.