N-acetylglucosaminyltransferase Vb coding sequences, recombinant cells and methods
View Patent ↗A previously unknown mammalian UDP-N-acetylglucosamine:α-6-D-mannoside β-1,6-N-acetylglucosaminyl-transferase (termed GlcNAc T-Vb herein) coding sequence, protein, recombinant host cells and antibodies which specifically bind GlcNAc T-Vb are described. GlcNAc T-Vb is encoded on human chromosome 17, whereas the prior GlcNAc T-V is encoded on human chromosome 2.
1. An isolated DNA molecule comprising a nucleotide sequence encoding a polypeptide having N-acetylglucosaminyl transferase V activity, said nucleotide sequence having at least 95% homology with a nucleotide sequence given in SEQ ID NO:7.
2. The DNA molecule of claim 1 , wherein said nucleotide sequence encodes human GlcNAc T-Vb.
3. The DNA molecule of claim 2 , wherein said nucleotide sequence encodes a polypeptide having the amino acid sequence given in SEQ ID NO:8.
4. The DNA molecule of claim 3 , wherein said nucleotide sequence is given in SEQ ID NO:7.
5. A DNA molecule comprising the DNA sequence of claim 1 and further comprising an exogenous nucleotide sequence.
6. The DNA molecule of claim 5 , wherein said exogenous nucleotide sequence is an expression vector.
7. An isolated recombinant host cell comprising the DNA molecule of claim 1 .
8. The recombinant cell of claim 7 , wherein said cell is a bacterial cell.
9. The recombinant cell of claim 8 , wherein said bacterial cell is Escherichia coli.
10. The recombinant cell of claim 7 , wherein said cell is a mammalian cell.
11. The recombinant cell of claim 10 , wherein said cell is selected from the group consisting of a COS-7 cell, a HEK-293 cell and a 3T3 cell.
12. The recombinant cell of claim 7 , wherein said cell is an insect cell, a yeast cell or a fungal cell.
13. An isolated recombinant host cell comprising the DNA molecule of claim 3 .
14. The recombinant cell of claim 13 , wherein said cell is a bacterial cell.
15. The recombinant cell of claim 14 , wherein said bacterial cell is Escherichia coli.
16. The recombinant cell of claim 13 , wherein said cell is a mammalian cell.
17. The recombinant cell of claim 16 , wherein said cell is selected from the group consisting of a COS-7 cell, a HEK-293 cell and a 3T3 cell.
18. The recombinant cell of claim 13 , wherein said cell is an insect cell, a yeast cell or a fungal cell.
19. A method of producing a polypeptide having N-Acetylglucosaminyl transferase V-b activity, said method comprising the step of culturing the recombinant cell of claim 7 under conditions for expression of said GlcNAc T-Vb.
20. A method of producing a polypeptide having N-Acetylglucosaminyl transferase V-b activity, said method comprising the step of culturing the recombinant cell of claim 13 under conditions for expression of said GlcNAc T-Vb.