G-coupled receptor showing selective affinity for ATP
The present invention relates to an isolated G-protein coupled receptor, nucleic acid sequence encoding the receptor, and host cells capable of expressing the receptor. The invention further comprises methods for detecting the expression of a G-protein coupled receptor, and methods for identifying agonists or antagonists of the receptor. The invention still further encompasses methods for preparing an isolated G-protein coupled receptor.
1. An isolated receptor comprising an amino acid sequence that is at least 95% identical to amino acid residues 424-795 of SEQ ID NO: 1.
2. The receptor according to claim 1 having a selective affinity ATP.
3. A composition comprising the isolated receptor of claim 1 .
4. A kit comprising the isolated receptor of claim 1 .
5. A method of preparing the receptor of claim 1 , which comprises:
a) inserting a vector encoding the receptor of claim 1 in a suitable host cell;
b) incubating the cell under conditions allowing the expression of the receptor;
c) recovering the receptor; and
d) purifying the recovered receptor, thereby preparing an isolated receptor according to claim 1 .
6. An isolated receptor comprising amino acid residues 424-795 of SEQ ID NO: 1.