Crystallization of FMS-like tyrosine kinase 3
Provided are crystals and structure coordinates relating to FMS-like tyrosine kinase 3 and its various uses.
1. A composition comprising a protein in crystalline form wherein the protein consists of SEQ ID NO:3, wherein said protein is in complex with a kinase inhibitor ligand, and wherein the protein crystal has a crystal lattice in a P4 2 2 1 2 space group and unit cell dimensions, +/−5%, of a=146.278 Å, b=146.278 Å, c=100.946 Å, α=β=γ=90°.
2. A composition according to claim 1 wherein the crystal diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
3. A method for forming a crystal of a protein comprising:
forming a crystallization volume comprising: a precipitant solution and a protein wherein the protein consists of SEQ ID NO:3, wherein said protein is in complex with a kinase inhibitor ligand, and wherein the protein crystal has a crystal lattice in a P4 2 2 1 2 space group and unit cell dimensions, +/−5%, of a=146.278 Å, b=146.278 Å, c=100.946 Å, α=β=γ=90°; and
storing the crystallization volume under conditions suitable for crystal formation of the protein.
4. A method according to claim 3 wherein the crystal diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
5. A method according to claim 3 , the method further comprising diffracting the protein crystal to produce a diffraction protein and solving the structure of the protein from the diffraction pattern.
6. An isolated soluble protein consisting of residues 564-713 and 778-954 of SEQ ID NO:1.
7. The protein according to claim 6 where the protein is expressed from a nucleic acid molecule that consists of SEQ ID NO:2.
8. A soluble protein consisting of SEQ ID NO:3.
9. An isolated non-crystalline protein consisting of amino acids 564-713 and 778-954 of SEQ ID NO:1.
10. A non-crystalline protein consisting of SEQ ID NO:3.