Dark-field laser-scattering microscope for analyzing single macromolecules
To further isolate the scattered light 112 of a specific particle from the scattered light of a different particle, a pin hole device 116 can be used. The pin hole device 116 prevents unfocused light from other particles from entering the second plane 118 , thus isolating a desired, focused image of one particle 110 of the sample 106 . In one embodiment, the pin hole 116 has a size of several microns. At the second plane 118 , a traditional imaging detection device 120 detects the defocused light. The position of the second image plane 118 is chosen for good angular resolution of the scattered light 112 , and at the same time gives enough light for the imaging detection device 120 to obtain a sufficient reading.
1. A microscope for analyzing single macromolecules comprising:
a variable wavelength, movable, collimated laser mounted above a sample and directed at the sample to illuminate the sample;
a series of imaging optics to focus scattered light from the sample onto an imaging plane; and
a detector to detect the defocusing scattered light of the sample at a second plane.
2. The microscope of claim 1 , wherein a pin hole screen is placed at the imaging plane between the sample and the detector to limit the unwanted scattered light that passes through to the second plane.
3. The microscope of claim 1 , wherein the detector is selected from a group consisting of a charge-coupled device (CCD) or Complementary Metal Oxide Semiconductor (CMOS) two-dimensional imaging detector.
4. The microscope of claim 1 , wherein a data processing device compiles the defocused scattered light of the sample at a second plane from the detector to determine the characteristics of the sample, including a three dimensional image, size, molecular weight, and refractive index.
5. A microscope for analyzing single macromolecules comprising:
a variable wavelength, movable, collimated laser mounted above a sample and directed at the sample to illuminate the sample;
a series of imaging optics to focus scattered light from the sample onto an imaging plane;
a detector to detect the defocusing scattered light of the sample at a second plane;
a pin hole screen placed at the imaging plane between the sample and the detector to limit the unwanted scattered light that passes through to the second plane;
the detector selected from a group consisting of a charge-coupled device (CCD) or Complementary Metal Oxide Semiconductor (CMOS) two-dimensional imaging detector; and
a data processing device to compile the data from the detector to determine the characteristics of the sample, including a three-dimensional image, size, molecular weight, and refractive index.
6. A method for single macromolecule in-vivo microanalysis, the method comprising acts of:
directing a variable wavelength, movable, collimated laser at a sample from a well-defined angle;
focusing the scattered light from the sample through a series of imaging optics and onto an imaging plane;
detecting the defocusing scattered light at a second plane with a detector;
altering the angle of the laser to collect scattered light from the same area of the sample; and
altering the wavelength of the laser to collect scattered light from the same area of the sample.
7. The method of claim 6 , further comprising the act of compiling data from the detector with a data processing device to determine the characteristics of the sample, including a three-dimensional image, size, molecular weight, and refractive index.
8. The method of claim 6 , comprising the additional act of placing a pin hole screen between the sample and the detector to limit the scattered light that passes through to the second plane.
9. The method of claim 8 , further comprising the act of moving the pin hole screen to allow scattered light from different particles to pass through to the second plane.
10. The method of claim 6 , further comprising the act of moving the sample to cause the laser to illuminate a different area of the sample.
11. The method of claim 6 , further comprising the act of moving the laser along with the series of imaging optics to illuminate a different area of the sample.
12. The method of claim 6 , wherein the detector is selected from a group consisting of a charge-coupled device (CCD) or Complementary Metal Oxide Semiconductor (CMOS) two-dimensional imaging detector.
13. A method for single macromolecule in-vivo microanalysis, the method comprising acts of:
directing a variable wavelength, movable, collimated laser at a sample from a well-defined angle;
focusing the scattered light from the sample through a series of imaging optics and onto an imaging plane;
detecting the defocusing scattered light at a second plane with a detector, the detector being selected from a group consisting of a charge-coupled device (CCD) or Complementary Metal Oxide Semiconductor (CMOS) two-dimensional imaging detector;
altering the angle of the laser to collect scattered light from the same area of the sample;
altering the wavelength of the laser to collect scattered light from the same area of the sample;
compiling data from the detector with a data processing device to determinethe characteristics of the sample, including a three-dimensional image, size, molecular weight, and refractive index;
placing a pin hole screen between the sample and the detector to limit the scattered light that passes through to the second plane;
moving the pin hole screen to allow scattered light from different particles topass through to the second plane;
moving the sample to cause the laser to illuminate a different area of the sample; and
moving the laser along with the series of imaging optics to illuminate a different area of the sample.